Glut-1 antibody
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Rabbit anti Human Glut-1 (C-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Glut-1
- Region
- (C-TERMINAL)
Antibody Quality and 
Unrivaled 
| Rabbit anti GLUT-1 antibody recognises an epitope within the C-Terminal (CT) region of GLUT-1 (Glucose transporter 1), a ubiquitously expressed integral membrane glycoprotein, and member of the major facilitator superfamily, which plays a major role in the transport of glucose across the cell membrane, and the blood-brain barrier. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human GLUT-1 (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Keyhole limpet haemocyanin conjugated synthetic peptide sequence C-ELFHPLGADSQV from the C-terminal region of GLUT-1 (NM_006516).
- Approx. Protein Concentrations
- Ig concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Paraffin 1 | |
1;100 | |
| Western Blotting | |
1:500 | 1:2000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Colorectal carcinoma
- Western Blotting
- AHP2173 detects a band of approximately 54kDa in human Jurkat cell lysates.
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- Synonyms
- Glucose Transporter 1
- RRID
- AB_10672762
- UniProt
- P11167
- P11166
- P17809
- Entrez Gene
- SLC2A1
- Slc2a1
- Slc2a1
- GO Terms
- GO:0016021 integral to membrane
- GO:0005624 membrane fraction
- GO:0005975 carbohydrate metabolic process
- GO:0006112 energy reserve metabolic process
- GO:0006767 water-soluble vitamin metabolic process
- GO:0050796 regulation of insulin secretion
- GO:0030496 midbody
- GO:0033300 dehydroascorbic acid transporter activity
- GO:0042470 melanosome
- View More GO Terms
- GO:0055056 D-glucose transmembrane transporter activity
- GO:0005351 sugar:hydrogen symporter activity
- GO:0005901 caveola
- GO:0005911 cell-cell junction
- GO:0006970 response to osmotic stress
- GO:0019900 kinase binding
- GO:0042910 xenobiotic transporter activity
- GO:0016323 basolateral plasma membrane
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