Glut-1 antibody

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Rabbit anti Human Glut-1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2173 E P* WB 50 µg
Rabbit anti GLUT-1 antibody recognises an epitope within the C-Terminal (CT) region of GLUT-1 (Glucose transporter 1), a ubiquitously expressed integral membrane glycoprotein, and member of the major facilitator superfamily, which plays a major role in the transport of glucose across the cell membrane, and the blood-brain barrier.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human GLUT-1 (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Keyhole limpet haemocyanin conjugated synthetic peptide sequence C-ELFHPLGADSQV from the C-terminal region of GLUT-1 (NM_006516).
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P11167 Related reagents
P11166 Related reagents
P17809 Related reagents
Entrez Gene
SLC2A1 Related reagents
Slc2a1 Related reagents
Slc2a1 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0005975 carbohydrate metabolic process
GO:0006112 energy reserve metabolic process
GO:0006767 water-soluble vitamin metabolic process
GO:0050796 regulation of insulin secretion
GO:0030496 midbody
GO:0033300 dehydroascorbic acid transporter activity
GO:0042470 melanosome
GO:0055056 D-glucose transmembrane transporter activity
GO:0005351 sugar:hydrogen symporter activity
GO:0005901 caveola
GO:0005911 cell-cell junction
GO:0006970 response to osmotic stress
GO:0019900 kinase binding
GO:0042910 xenobiotic transporter activity
GO:0016323 basolateral plasma membrane
Regulatory
For research purposes only

Applications of Glut-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1 1;100
Western Blotting 1:500 1:2000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Colorectal carcinoma
Western Blotting
AHP2173 detects a band of approximately 54kDa in human Jurkat cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*