Glucosidase II Alpha antibody

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Rabbit anti Glucosidase II Alpha

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00679
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human glucosidase II alpha antibody recognizes the neutral alpha-glucosidase AB, also known as HEL-S-164nA, alpha glucosidase II alpha subunit, alpha-glucosidase 2, or epididymis secretory sperm binding protein Li 164nA or GANAB.

The GANAB gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease (provided by RefSeq, Jul 2016).

Rabbit anti Human glucosidase II alpha antibody detects a band of 107 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti glucosidase II alpha detects a band of approximately 107 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered glycine
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Recombinant protein encompassing part of the N-terminal region of human alpha glucosidase II alpha

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q14697
Entrez Gene
GANAB
GO Terms
GO:0005515 protein binding
GO:0006457 protein folding
GO:0030246 carbohydrate binding
GO:0005788 endoplasmic reticulum lumen
GO:0005794 Golgi apparatus
GO:0018279 protein N-linked glycosylation via asparagine
GO:0033919 glucan 1,3-alpha-glucosidase activity
GO:0042470 melanosome
GO:0043687 post-translational protein modification
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Glucosidase II Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml