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GLI2 antibody

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Anti GLI2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1
Anti GLI2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Rabbit anti Human GLI2 antibody (VPA00196) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 81 second exposure. Arrow points to GLI2 (molecular weight 191 kDa).

Rabbit anti GLI2

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
GLI2

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Product Code Applications Pack Size List Price Your Price Qty
VPA00196
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Rabbit anti Human GLI2 antibody recognizes GLI2, also known as GLI family zinc finger protein 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, oncogene GLI2, tax helper protein 1, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein and zinc finger protein GLI2.

The GLI2 gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by GLI2 localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B (provided by RefSeq, Jul 2008).

Rabbit anti Human GLI2 antibody detects a band of 191 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti GLI2 detects a band of approximately 191 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Synthetic peptide corresponding to amino acids 46-60 of human GLI2 (isoform a)
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P10070
Entrez Gene
GLI2
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005515 protein binding
GO:0001501 skeletal system development
GO:0001649 osteoblast differentiation
GO:0001822 kidney development
GO:0002076 osteoblast development
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005634 nucleus
GO:0007411 axon guidance
GO:0007442 hindgut morphogenesis
GO:0007507 heart development
GO:0008270 zinc ion binding
GO:0008283 cell proliferation
GO:0009913 epidermal cell differentiation
GO:0009954 proximal/distal pattern formation
GO:0016563 transcription activator activity
GO:0021508 floor plate formation
GO:0021696 cerebellar cortex morphogenesis
GO:0021775 smoothened signaling pathway involved in ventral spinal cord interneuron specification
GO:0021965 spinal cord ventral commissure morphogenesis
GO:0021983 pituitary gland development
GO:0030324 lung development
GO:0033089 positive regulation of T cell differentiation in thymus
GO:0042475 odontogenesis of dentine-containing tooth
GO:0045740 positive regulation of DNA replication
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048566 embryonic digestive tract development
GO:0048589 developmental growth
GO:0048754 branching morphogenesis of a tube

VPA00196

060815

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