GLI2 antibody
Rabbit anti GLI2
GLI2 antibody recognizes the zinc finger protein GLI2, also known as GLI family zinc finger protein 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, oncogene GLI2, or tax helper protein 1.
GLI2 Antibody with Control Lysate
GLI2 antibody recognizes the zinc finger protein GLI2, also known as GLI family zinc finger protein 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, oncogene GLI2, or tax helper protein 1.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00196 | WB | 100 µl | ||||
| VPA00196KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human GLI2 antibody recognizes GLI2, also known as GLI family zinc finger protein 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, oncogene GLI2, tax helper protein 1, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein and zinc finger protein GLI2.
The GLI2 gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by GLI2 localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B (provided by RefSeq, Jul 2008).
Rabbit anti Human GLI2 antibody detects a band of 191 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The GLI2 gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by GLI2 localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B (provided by RefSeq, Jul 2008).
Rabbit anti Human GLI2 antibody detects a band of 191 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti GLI2 detects a band of approximately 191 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Synthetic peptide corresponding to amino acids 46-60 of human GLI2 (isoform a)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P10070 Related reagents
- Entrez Gene
- GLI2 Related reagents
- GO Terms
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0005515 protein binding
- GO:0001501 skeletal system development
- GO:0001649 osteoblast differentiation
- GO:0001822 kidney development
- GO:0002076 osteoblast development
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005634 nucleus
- GO:0007411 axon guidance
- GO:0007442 hindgut morphogenesis
- GO:0007507 heart development
- GO:0008270 zinc ion binding
- GO:0008283 cell proliferation
- GO:0009913 epidermal cell differentiation
- GO:0009954 proximal/distal pattern formation
- GO:0016563 transcription activator activity
- GO:0021508 floor plate formation
- GO:0021696 cerebellar cortex morphogenesis
- GO:0021775 smoothened signaling pathway involved in ventral spinal cord interneuron specification
- GO:0021965 spinal cord ventral commissure morphogenesis
- GO:0021983 pituitary gland development
- GO:0030324 lung development
- GO:0033089 positive regulation of T cell differentiation in thymus
- GO:0042475 odontogenesis of dentine-containing tooth
- GO:0045740 positive regulation of DNA replication
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048566 embryonic digestive tract development
- GO:0048589 developmental growth
- GO:0048754 branching morphogenesis of a tube
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of GLI2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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