GFAP antibody

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Anti Human GFAP (C-Terminal) Antibody thumbnail image 1

Anti Human GFAP (C-Terminal) Antibody thumbnail image 2

Anti Human GFAP (C-Terminal) Antibody thumbnail image 3

Anti Human GFAP (C-Terminal) Antibody gallery image 1 — Western blotting analysis of human cerebellum (A), Mouse brain (B) and Rat brain (C) lysates (35µg protein in RIPA buffer) probed with Goat anti Human GFAP antibody (**AHP1468**) at 0.01, 0.3 and 0.01μgml^-1 respectively. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Anti Human GFAP (C-Terminal) Antibody gallery image 2 — Immunohistochemical staining of formalin fixed cryosection of human hypothalamus following antigen retrieval with citrate buffer pH 6.0 at 80°C for 30 min. Sections stained with Goat anti Human GFAP (**AHP1468**) at 0.01 μgml^-1 followed by secondary anti Goat-HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

Anti Human GFAP (C-Terminal) Antibody gallery image 3 — Immunohistochemical staining of paraffin embedded human cerebellum following heat mediated antigen retrieval in citrate buffer pH 6.0 with Goat anti Human GFAP antibody at 2μgml^-1 followed by secondary anti Goat-HRP and DAB chromogen visualization

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Goat anti Human GFAP (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: GFAP
Region: (C-TERMINAL)

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References (1)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

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AHP1468 Datasheet Datasheet SDS SDS	E WB	0.1 mg		Log in	Get Quote
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Goat anti Human GFAP antibody recognizes an epitope within the C-terminal (CT) region of human GFAP (glial fibrillary acidic protein), a class III intermediate filament (IF) protein specifically expressed by glial cells or cells of glial origin e.g astrocytes, ependymal cells and Schwann cells.

GFAP plays a role in several cellular functions within the central nervous system (CNS), including cell structure and stability, communication, motility and mitosis, and is rapidly synthesized during astrogliosis, following trauma/injury. Mutations in the GFAP gene are responsible for the rare autosomal dominant disorder known as Alexander disease, resulting in the destruction of brain white matter and the formation of fibrous, eosinophilic deposits known as Rosenthal fibers. Characteristics of this disease are associated with transgenes and other mutation types in mouse.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat Expected from Sequence

Dog Expected from Sequence; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to human GFAP (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: TRIS buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: Peptide sequence C-DGEVIKESKQEHKD from the C-terminal region of GFAP (NP_002046.1).
Approx. Protein Concentrations: IgG concentration 0.5mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse
Rat	Expected from Sequence
Dog	Expected from Sequence

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/32000
Western Blotting		1.0	3.0ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1468 detects a band of approximately 48kDa in mouse brain cell lysates.

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