GFAP antibody
Goat anti Human GFAP antibody recognizes an epitope within the C-terminal (CT) region of human GFAP (glial fibrillary acidic protein), a class III intermediate filament (IF) protein specifically expressed by glial cells or cells of glial origin e.g astrocytes, ependymal cells and Schwann cells.
GFAP plays a role in several cellular functions within the central nervous system (CNS), including cell structure and stability, communication, motility and mitosis, and is rapidly synthesized during astrogliosis, following trauma/injury. Mutations in the GFAP gene are responsible for the rare autosomal dominant disorder known as Alexander disease, resulting in the destruction of brain white matter and the formation of fibrous, eosinophilic deposits known as Rosenthal fibers. Characteristics of this disease are associated with transgenes and other mutation types in mouse.
GFAP plays a role in several cellular functions within the central nervous system (CNS), including cell structure and stability, communication, motility and mitosis, and is rapidly synthesized during astrogliosis, following trauma/injury. Mutations in the GFAP gene are responsible for the rare autosomal dominant disorder known as Alexander disease, resulting in the destruction of brain white matter and the formation of fibrous, eosinophilic deposits known as Rosenthal fibers. Characteristics of this disease are associated with transgenes and other mutation types in mouse.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Expected from Sequence Dog Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human GFAP (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence C-DGEVIKESKQEHKD from the C-terminal region of GFAP (NP_002046.1).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
Applications of GFAP antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/32000 | ||
| Western Blotting | 1.0 | 3.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1468 detects a band of approximately 48kDa in mouse brain cell lysates.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
Further Reading
-
Gomes, F.C. et al. (1999) Glial fibrillary acidic protein (GFAP): modulation by growth factors and its implication in astrocyte differentiation.
Braz J Med Biol Res. 32 (5): 619-31.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
