GATA3 antibody

Rabbit anti GATA3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
GATA3

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AHP2403
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IC IF P * WB 50 µg loader
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Rabbit anti GATA3 antibody recognizes GATA3, also known as Trans-acting T-cell-specific transcription factor GATA-3 or GATA-binding factor 3. The protein is a member of the GATA family of transcription factors, which has received its name from binding DNA sequences containing the GATA consensus motif 5'-[AT]GATA[AG]-3'  (uniprot P15976).

GATA3 has been shown to play a critical role in T cell development and the differentiation of T helper 2 (Th2) cells (Ho et al.1991). In addition, GATA3 has been implied to have tissue specific functions such as a regulatory role in the development of the mammary gland epithelium and skin tissue (Chou et al. 2010).

Aberrant GATA3 levels have been identified in a variety of cancers including breast cancer. Indeed, GATA3 has become a marker for certain breast cancer types and GATA3 antibodies are commonly used for the immunohistochemical evaluation of breast cancer samples (Krings et al. 2014).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to GATA3 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
1 mg/ml Bovine Serum Albumin
30% Glycerol
Immunogen
Purified partial recombinant GATA3 protein expressed in E. coli
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry 1/50 1/500
Immunofluorescence 1/50 1/500
Immunohistology - Paraffin 1 1/50 1/500
Western Blotting 1/1000
  1. 1This antibody requires heat-mediated antigen retrieval prior to staining. Bio-Rad recommend citrate buffer pH6.0 for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti GATA3 antibody recognizes a band of approximately 55 kDa in SH-SY5Y cell lysate and SH-SY5Y nuclear extract

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Synonyms
GATA-3
GATA-Binding Factor 3
UniProt
P23771
Entrez Gene
GATA3
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006952 defense response
GO:0007596 blood coagulation
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006355 regulation of transcription, DNA-dependent
GO:0006366 transcription from RNA polymerase II promoter
GO:0009653 anatomical structure morphogenesis
GO:0008134 transcription factor binding
GO:0008270 zinc ion binding
GO:0043565 sequence-specific DNA binding
GO:0043627 response to estrogen stimulus
GO:0071837 HMG box domain binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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