GATA1 antibody

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Anti GATA1 Antibody gallery image 1 — Immunofluorescent analysis of GATA1 (green) in A431 cells (right) using Rabbit anti GATA1 antibody (**AHP2401**) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GATA1 polyclonal antibody in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated cells were counterstained with phalloidin (F-actin, red) and Hoechst 33342 (Nucleus, blue). Images were taken at a magnification of 60x

Anti GATA1 Antibody gallery image 2 — Immunofluorescent analysis of GATA1 (green) in U251 cells (right) using Rabbit anti GATA1 antibody (**AHP2401**) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GATA1 polyclonal antibody in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated cells were counterstained with phalloidin (F-actin, red) and Hoechst 33342 (Nucleus, blue). Images were taken at a magnification of 60x

Anti GATA1 Antibody gallery image 3 — Immunohistochemistry analysis of GATA1 using Rabbit anti GATA1 antibody (**AHP2401**) showing staining in the nucleus of paraffin-embedded human breast tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H₂O₂-methanol for 15 min at room temperature, washed with ddH₂O and PBS, and then probed with GATA1 polyclonal antibody diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting

Anti GATA1 Antibody gallery image 4 — Immunohistochemistry analysis of GATA1 showing staining in the nucleus of paraffin-embedded human brain tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H₂O₂-methanol for 15 min at room temperature, washed with ddH₂O and PBS, and then probed with GATA1 polyclonal antibody diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting

Anti GATA1 Antibody gallery image 5 — Immunohistochemistry analysis of GATA1 showing staining in the nucleus of paraffin-embedded human tonsil tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H₂O₂-methanol for 15 min at room temperature, washed with ddH₂O and PBS, and then probed with GATA1 polyclonal antibody diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting

Anti GATA1 Antibody gallery image 6 — Western blot analysis of GATA1 was performed by loading 25 ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with rabbit polyclonal antibody recognizing GATA1 at a dilution of 1/500 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1% Tween 20 and probed with goat anti-rabbit-HRP secondary antibody for at least one hour. Membranes were washed and detection was carried out using chemiluminescence

Rabbit anti GATA1

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP2401	IC IF P* WB			50 µg

Rabbit anti GATA1 antibody recognizes GATA1, also known as GATA binding factor 1. The protein is a member of the GATA family of transcription factors, which has received its name from binding DNA containing the GATA consensus motif 5'-[AT]GATA[AG]-3' (uniprot P15976).

GATA1 acts as an erythroid transcription factor by regulating erythroid-specific genes. In addition to that, GATA1 has also been implied to play a role in regulating differentiation of other hematopoietic lineages (including mast cells and megakaryocytes) and endothelial cells (Orkin 1992).

GATA1 alterations have been implicated in a variety of diseases, including acute megakaryoblastic leukemia (AMKL) associated with Trisomy 21 and thrombocytopenia (Ciovacco et al. 2008).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to GATA1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.05% Sodium Azide (NaN₃)
1 mg/ml Bovine Serum Albumin
30% Glycerol

Immunogen

N-terminal truncated human recombinant protein

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch

More Information

UniProt: P15976 Related reagents
Entrez Gene: GATA1 Related reagents
GO Terms: GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0007596 blood coagulation; GO:0005654 nucleoplasm; GO:0005730 nucleolus; GO:0006357 regulation of transcription from RNA polymerase II promoter; GO:0007275 multicellular organismal development; GO:0008270 zinc ion binding; GO:0031965 nuclear membrane
Regulatory: For research purposes only

Applications of GATA1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunocytochemistry	1/50	1/500
Immunofluorescence	1/50	1/500
Immunohistology - Paraffin ¹	1/50	1/500
Western Blotting		1/500

¹This antibody requires heat-mediated antigen retrieval prior to staining. Bio-Rad recommend citrate buffer pH 6.0 for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Rabbit anti GATA1 antibody recognizes a band of approximately 43 kDa in NCCT cell lysate, NTERRA cell lysate and K562 cell lysate.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*

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