GATA1 antibody






GATA1 acts as an erythroid transcription factor by regulating erythroid-specific genes. In addition to that, GATA1 has also been implied to play a role in regulating differentiation of other hematopoietic lineages (including mast cells and megakaryocytes) and endothelial cells (Orkin 1992).
GATA1 alterations have been implicated in a variety of diseases, including acute megakaryoblastic leukemia (AMKL) associated with Trisomy 21 and thrombocytopenia (Ciovacco et al. 2008).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to GATA1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
1 mg/ml Bovine Serum Albumin
30% Glycerol - Immunogen
- N-terminal truncated human recombinant protein
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P15976
- Entrez Gene
- GATA1
- GO Terms
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0007596 blood coagulation
- GO:0005654 nucleoplasm
- GO:0005730 nucleolus
- GO:0006357 regulation of transcription from RNA polymerase II promoter
- GO:0007275 multicellular organismal development
- GO:0008270 zinc ion binding
- GO:0031965 nuclear membrane
- Regulatory
- For research purposes only
Applications of GATA1 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunocytochemistry | 1/50 | 1/500 | |
Immunofluorescence | 1/50 | 1/500 | |
Immunohistology - Paraffin 1 | 1/50 | 1/500 | |
Western Blotting | 1/500 |
- 1This antibody requires heat-mediated antigen retrieval prior to staining. Bio-Rad recommend citrate buffer pH 6.0 for this purpose.
- Western Blotting
- Rabbit anti GATA1 antibody recognizes a band of approximately 43 kDa in NCCT cell lysate, NTERRA cell lysate and K562 cell lysate.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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