GATA1 antibody

Rabbit anti GATA1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
GATA1

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AHP2401
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SDS Safety Datasheet SDS
IC IF P * WB 50 µg loader
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Rabbit anti GATA1 antibody recognizes GATA1, also known as GATA binding factor 1. The protein is a member of the GATA family of transcription factors, which has received its name from binding DNA containing the GATA consensus motif 5'-[AT]GATA[AG]-3'  (uniprot P15976).

GATA1 acts as an erythroid transcription factor by regulating erythroid-specific genes. In addition to that, GATA1 has also been implied to play a role in regulating differentiation of other hematopoietic lineages (including mast cells and megakaryocytes) and endothelial cells (Orkin 1992).

GATA1 alterations have been implicated in a variety of diseases, including acute megakaryoblastic leukemia (AMKL) associated with Trisomy 21 and thrombocytopenia (Ciovacco et al. 2008).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to GATA1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
1 mg/ml Bovine Serum Albumin
30% Glycerol
Immunogen
N-terminal truncated human recombinant protein
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry 1/50 1/500
Immunofluorescence 1/50 1/500
Immunohistology - Paraffin 1 1/50 1/500
Western Blotting 1/500
  1. 1This antibody requires heat-mediated antigen retrieval prior to staining. Bio-Rad recommend citrate buffer pH 6.0 for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti GATA1 antibody recognizes a band of approximately 43 kDa in NCCT cell lysate, NTERRA cell lysate and K562 cell lysate.

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Synonyms
Eryf1
GATA-1
GATA-binding Factor 1
UniProt
P15976
Entrez Gene
GATA1
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0007596 blood coagulation
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0007275 multicellular organismal development
GO:0008270 zinc ion binding
GO:0031965 nuclear membrane

AHP2401

160223

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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