Galectin-3 antibody

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Rabbit anti Galectin-3

Galectin-3 antibody recognizes galectin-3, also known as gal-3, 35 kDa lectin, carbohydrate-binding protein 35, CBP 35, galactose-specific lectin 3, galactoside-binding protein, GALBP, or mac-2 antigen. The antibody has been extensively validated for western blotting using whole cell lysates.

Galectin-3 Antibody with Control Lysate

Galectin-3 antibody recognizes galectin-3, also known as gal-3, 35 kDa lectin, carbohydrate-binding protein 35, CBP 35, galactose-specific lectin 3, galactoside-binding protein, GALBP, IgE-binding protein, L-31, laminin-binding protein, lectin L-29 or mac-2 antigen.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00076 WB 100 µl
VPA00076KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human galectin-3 antibody recognizes human galectin-3, a member of the galectin family of beta-galactoside-binding proteins which are involved in both intracellular and extracellular functions and exhibit anti-apoptotic properties.

Galectin-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Galectin-3 acts as a regulator of tumor metastasis, and several studies have shown a correlation between levels of galectin-3 and tumor progression in liver, thyroid, colon, gastric and breast carcinomas, making galectin-3 an emerging cancer marker.

Rabbit anti Human galectin-3 antibody detects a single band of 26 kDa in a range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Rabbit anti galectin-3 detects a band of approximately 26 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human galectin-3
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg MCF7 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P17931 Related reagents
Entrez Gene
LGALS3 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005529 sugar binding
GO:0005634 nucleus
GO:0005743 mitochondrial inner membrane
GO:0019863 IgE binding
GO:0030154 cell differentiation
GO:0045087 innate immune response
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Galectin-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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