Galectin-3 antibody
KO Validated
Filter by Application:
WB ResetRabbit anti Human Galectin-3:Biotin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Format
- Purified
- Specificity
- Galectin-3
Antibody Quality and Performance Guarantee
| Rabbit anti Human Galectin-3 antibody recognizes human Galectin-3 (Gal-3), also known as the Mac-2 antigen. Gal-3 is a 250 amino acid ~35 kDa member of the galectin family of beta-galactoside-binding proteins, which is involved in both intracellular and extracellular functions and exhibits anti-apoptotic properties. Gal-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Gal-3 acts as a regulator of tumor metastasis expression correlates with tumor progression in liver, thyroid, colon, gastric and breast carcinomas (Califice et al. 2004). |
|
- Target Species
- Human
- Western Blotting
- AHP1481B may be used under either reducing or non-reducing conditions with PHP188 as the positive control.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to human galectin-3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human galectin-3.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.25 | 1.0ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Recombinant Human Galectin-3 | PHP188 | E FN WB | 50 µg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Recombinant Human Galectin-3 | ||||||
References for Galectin-3 antibody
-
Chen, Y.X. et al. (2015) The Sirt1 activator SRT1720 attenuates angiotensin II-induced atherosclerosis in apoE⁻/⁻ mice through inhibiting vascular inflammatory response.
Biochem Biophys Res Commun. 465 (4): 732-8.
Further Reading
-
Dumic, J. et al. (2006) Galectin-3: an open-ended story.
Biochim Biophys Acta. 1760 (4): 616-35.
- Synonyms
- MAC-2
- RRID
- AB_1102581
- UniProt
- P17931
- Entrez Gene
- LGALS3
- GO Terms
- GO:0005886 plasma membrane
- GO:0005529 sugar binding
- GO:0005634 nucleus
- GO:0005743 mitochondrial inner membrane
- GO:0019863 IgE binding
- GO:0030154 cell differentiation
- GO:0045087 innate immune response
AHP1481B
155737
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with GALECTIN specificity
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