Galectin-3 antibody

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Rabbit anti Human Galectin-3:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1481B E WB 50 µg
Rabbit anti Human Galectin-3 antibody recognizes human Galectin-3 (Gal-3), also known as the Mac-2 antigen. Gal-3 is a 250 amino acid ~35 kDa member of the galectin family of beta-galactoside-binding proteins, which is involved in both intracellular and extracellular functions and exhibits anti-apoptotic properties.

Gal-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Gal-3 acts as a regulator of tumour metastasis, and several studies have shown a correlation between levels of Gal-3 and tumour progression in liver, thyroid, colon, gastric and breast carcinomas, making Gal-3 an emerging cancer marker.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - lyophilised
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to human galectin-3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human galectin-3.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P17931 Related reagents
Entrez Gene
LGALS3 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005529 sugar binding
GO:0005634 nucleus
GO:0005743 mitochondrial inner membrane
GO:0019863 IgE binding
GO:0030154 cell differentiation
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of Galectin-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1481B may be used under either reducing or non-reducing conditions with PHP188 as the positive control.

Positive Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Recombinant Human Galectin-3 PHP188 50 µg E FN WB

Product Specific References

References for Galectin-3 antibody

  1. Chen, Y.X. et al. (2015) The Sirt1 activator SRT1720 attenuates angiotensin II-induced atherosclerosis in apoE⁻/⁻ mice through inhibiting vascular inflammatory response.
    Biochem Biophys Res Commun. 465 (4): 732-8.

Further Reading

  1. Dumic, J. et al. (2006) Galectin-3: an open-ended story.
    Biochim Biophys Acta. 1760 (4): 616-35.