Galectin-3 antibody
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Rabbit anti Human Galectin-3 antibody recognizes human Galectin-3 (Gal-3), also known as the Mac-2 antigen. Gal-3 is a 250 amino acid ~35 kDa member of the galectin family of beta-galactoside-binding proteins, which is involved in both intracellular and extracellular functions and exhibits anti-apoptotic properties.
Gal-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Gal-3 acts as a regulator of tumour metastasis, and several studies have shown a correlation between levels of Gal-3 and tumour progression in liver, thyroid, colon, gastric and breast carcinomas, making Gal-3 an emerging cancer marker.
Gal-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Gal-3 acts as a regulator of tumour metastasis, and several studies have shown a correlation between levels of Gal-3 and tumour progression in liver, thyroid, colon, gastric and breast carcinomas, making Gal-3 an emerging cancer marker.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to human galectin-3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant human galectin-3.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P17931 Related reagents
- Entrez Gene
- LGALS3 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0005529 sugar binding
- GO:0005634 nucleus
- GO:0005743 mitochondrial inner membrane
- GO:0019863 IgE binding
- GO:0030154 cell differentiation
- GO:0045087 innate immune response
- Regulatory
- For research purposes only
Applications of Galectin-3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.25 | 1.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1481B may be used under either reducing or non-reducing conditions with PHP188 as the positive control.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Positive Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Recombinant Human Galectin-3 | PHP188 | 50 µg | E FN WB |
Product Specific References
References for Galectin-3 antibody
-
Chen, Y.X. et al. (2015) The Sirt1 activator SRT1720 attenuates angiotensin II-induced atherosclerosis in apoE⁻/⁻ mice through inhibiting vascular inflammatory response.
Biochem Biophys Res Commun. 465 (4): 732-8.
Further Reading
-
Dumic, J. et al. (2006) Galectin-3: an open-ended story.
Biochim Biophys Acta. 1760 (4): 616-35.
