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|Goat anti Human GADD34 antibody recognizes the protein GADD34 (growth arrest and DNA-damage-inducible 34), also known as PPP1R15A. GADD34 is a member of a group of genes whose transcript levels are increased in response to treatment with DNA damaging agents or conditions of stress (Amundson et al. 1998). Induction of GADD34 by ionising radiation has been reported in some cell lines, regardless of p53 status (Hollander et al. 1997). Reports suggest that GADD34 may be involved in a signal transduction pathway controlling cell cycle arrest and/or apoptosis following DNA damage (Hollander et al. 2003).
Goat anti human GADD34 antibody has been used successfully for following GADD34 protein turnover in multiple cell lines and primary cells by Western blotting (Zhou et al. 2013)
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to GADD34 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.02% Sodium Azide 0.5% Bovine Serum Albumin
- Peptide sequence AAALDLSGRRG corresponding to the C-terminus of GADD34 (NP_055145).
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Western Blotting
- AHP683 detects a band of approximately 80-90kDa in U937 cell lysates. An overnight incubation time of 12 hours is recommended for this antibody.
|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Rabbit anti Goat IgG (Fc):FITC||STAR122F||F||1 mg||Log in|
|List Price||Your Price|
|Description||Rabbit anti Goat IgG (Fc):FITC|
|Rabbit anti Goat IgG (Fc):HRP||STAR122P||C E WB||1 mg||Log in|
|List Price||Your Price|
|Description||Rabbit anti Goat IgG (Fc):HRP|
References for GADD34 antibody
Zhou, W. et al. (2011) Association with endoplasmic reticulum promotes proteasomal degradation of GADD34 protein.
J Biol Chem. 286: 21687-96.
Zhou, W. et al. (2013) Phosphorylation at tyrosine 262 promotes GADD34 protein turnover.
J Biol Chem. 288 (46): 33146-55.
Imbeault, M. et al. (2009) Microarray study reveals that HIV-1 induces rapid type-I interferon-dependent p53 mRNA up-regulation in human primary CD4+ T cells.
Retrovirology. 6: 5.
Hollander, M.C. et al. (1997) Mammalian GADD34, an apoptosis- and DNA damage-inducible gene.
J Biol Chem. 272 (21): 13731-7.
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