Goat anti Human GADD34 antibody
recognizes the protein GADD34 (growth arrest and DNA-damage-inducible 34), also known as PPP1R15A. GADD34 is a member of a group of genes whose transcript levels are increased in response to treatment with DNA damaging agents or conditions of stress (Amundson et al. 1998
). Induction of GADD34 by ionising radiation has been reported in some cell lines, regardless of p53 status (Hollander et al. 1997
). Reports suggest that GADD34 may be involved in a signal transduction pathway controlling cell cycle arrest and/or apoptosis following DNA damage (Hollander et al. 2003
Goat anti human GADD34 antibody has been used successfully for following GADD34 protein turnover in multiple cell lines and primary cells by Western blotting (Zhou et al. 2013
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to GADD34 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
|0.5%||Bovine Serum Albumin|
- Peptide sequence AAALDLSGRRG corresponding to the C-terminus of GADD34 (NP_055145).
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
regulation of translation
response to DNA damage stimulus
cell cycle arrest
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of GADD34 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
- Western Blotting
- AHP683 detects a band of approximately 80-90kDa in U937 cell lysates. An overnight incubation time of 12 hours is recommended for this antibody.
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Secondary Antibodies Available
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Product Specific References
References for GADD34 antibody
Zhou, W. et al. (2011) Association with endoplasmic reticulum promotes proteasomal degradation of GADD34 protein.
J Biol Chem. 286: 21687-96.
Zhou, W. et al. (2013) Phosphorylation at tyrosine 262 promotes GADD34 protein turnover.
J Biol Chem. 288 (46): 33146-55.
Imbeault, M. et al. (2009) Microarray study reveals that HIV-1 induces rapid type-I interferon-dependent p53 mRNA up-regulation in human primary CD4+ T cells.
Retrovirology. 6: 5.
Hollander, M.C. et al. (1997) Mammalian GADD34, an apoptosis- and DNA damage-inducible gene.
J Biol Chem. 272 (21): 13731-7.
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