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GADD34 antibody

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Anti Human GADD34 Antibody thumbnail image 1 Anti Human GADD34 Antibody thumbnail image 2 Anti Human GADD34 Antibody thumbnail image 3
Anti Human GADD34 Antibody gallery image 1
Western blot analysis of HeLa whole cell lysate probed with Goat anti Human GADD34 (AHP683) followed by Donkey anti Sheep/Goat IgG:HRP.
Anti Human GADD34 Antibody gallery image 2
Western blotting analysis of HEPG2 cell lysate (35μg protein in RIPA buffer) probed with Goat anti Human GADD34 antibody (AHP683) at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Anti Human GADD34 Antibody gallery image 3
Immunohistochemical staining of paraffin embedded human liver probed with Goat anti Human GADD34 antibody (AHP683) following heat mediated antigen retrieval with citrate buffer pH 6.0, Detection with anti Goat secondary conjugated to HRP with DAB chromogen development

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WB C

Goat anti Human GADD34

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
GADD34

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Product Code Applications Pack Size List Price Your Price Qty
AHP683
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg
List Price Your Price

Goat anti Human GADD34 antibody recognizes the protein GADD34 (growth arrest and DNA-damage-inducible 34), also known as PPP1R15A. GADD34 is a member of a group of genes whose transcript levels are increased in response to treatment with DNA damaging agents or conditions of stress (Amundson et al. 1998). Induction of GADD34 by ionising radiation has been reported in some cell lines, regardless of p53 status (Hollander et al. 1997). Reports suggest that GADD34 may be involved in a signal transduction pathway controlling cell cycle arrest and/or apoptosis following DNA damage (Hollander et al. 2003).

Goat anti human GADD34 antibody has been used successfully for following GADD34 protein turnover in multiple cell lines and primary cells by Western blotting (Zhou et al. 2013)

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to GADD34 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Peptide sequence AAALDLSGRRG corresponding to the C-terminus of GADD34 (NP_055145).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5ug/ml 2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
Western Blotting
AHP683 detects a band of approximately 80-90kDa in U937 cell lysates. An overnight incubation time of 12 hours is recommended for this antibody.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

References for GADD34 antibody

  1. Zhou, W. et al. (2011) Association with endoplasmic reticulum promotes proteasomal degradation of GADD34 protein.
    J Biol Chem. 286: 21687-96.
  2. Zhou, W. et al. (2013) Phosphorylation at tyrosine 262 promotes GADD34 protein turnover.
    J Biol Chem. 288 (46): 33146-55.
  3. Imbeault, M. et al. (2009) Microarray study reveals that HIV-1 induces rapid type-I interferon-dependent p53 mRNA up-regulation in human primary CD4+ T cells.
    Retrovirology. 6: 5.

Further Reading

  1. Hollander, M.C. et al. (1997) Mammalian GADD34, an apoptosis- and DNA damage-inducible gene.
    J Biol Chem. 272 (21): 13731-7.

Western Blotting

RRID
AB_2168722
UniProt
O75807
Entrez Gene
PPP1R15A
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005783 endoplasmic reticulum
GO:0006417 regulation of translation
GO:0006974 response to DNA damage stimulus
GO:0007050 cell cycle arrest

AHP683

158230 270515

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