G3BP1 antibody

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Rabbit anti G3BP1

Rabbit anti-human G3BP1 antibody recognizes Ras GTPase-activating protein-binding protein 1, also known as ATP-dependent DNA helicase VIII, GAP SH3 domain-binding protein 1 or GAP binding protein.

G3BP1 Antibody with Control Lysate

Rabbit anti-human G3BP1 antibody recognizes Ras GTPase-activating protein-binding protein 1, also known as ATP-dependent DNA helicase VIII, GAP SH3 domain-binding protein 1 or GAP binding protein.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00492 WB 100 µl
VPA00492KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human G3BP1 antibody recognizes G3BP1 also known as, Ras GTPase-activating protein-binding protein 1, ATP-dependent DNA helicase VIII, G3BP-1, GAP SH3 domain-binding protein 1, GAP binding protein, Ras-GTPase-activating protein SH3-domain-binding protein or RasGAP-associated endoribonuclease G3BP.

G3BP1 gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of G3BP1 have been described, but the full-length nature of some of these variants has not been determined (provided by RefSeq, Jul 2008).

Rabbit anti Human G3BP1 antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
Preparation
20μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the N terminal region of human G3BP1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q13283 Related reagents
Entrez Gene
G3BP1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004003 ATP-dependent DNA helicase activity
GO:0004004 ATP-dependent RNA helicase activity
GO:0004519 endonuclease activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006810 transport
GO:0007265 Ras protein signal transduction
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of G3BP1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti G3BP1 detects a band of approximately 68 kDa in Jurkat cell lysates
Western Blotting
Anti G3BP1 detects a band of approximately 68 kDa in Jurkat cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB