FZD7 antibody

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Rabbit anti FZD7 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1850 P* 50 µg
Rabbit anti Human FZD7 antibody recognizes human Frizzled-7 (FZD7), a multi-pass membrane protein of 574 amino acids belonging to the G-protein coupled receptor FZ/Smo family.

FZD7 is a receptor for Wnt signaling proteins. FZD7 down regulates the functions of the tumor suppressor gene APC and thereby enhances beta catenin-mediated signals.

FZD7 is highly expressed adult skeletal muscle and foetal kidney. High expression is observed in the foetal lung, placenta, adult heart and brain tissues. It is specifically expressed in squamous cell oesophageal carcinomas.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human FZD7 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
An 18 amino acid synthetic peptide from the carboxyl terminus of human FZD7.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O75084 Related reagents
Entrez Gene
FZD7 Related reagents
GO Terms
GO:0001944 vasculature development
GO:0016021 integral to membrane
GO:0042813 Wnt receptor activity
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway
GO:0004930 G-protein coupled receptor activity
GO:0005737 cytoplasm
GO:0007199 G-protein signaling, coupled to cGMP nucleotide second messenger
GO:0007409 axonogenesis
GO:0007420 brain development
GO:0008406 gonad development
GO:0010551 regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010812 negative regulation of cell-substrate adhesion
GO:0014834 satellite cell maintenance involved in skeletal muscle regeneration
GO:0017147 Wnt-protein binding
GO:0030165 PDZ domain binding
GO:0032589 neuron projection membrane
GO:0033077 T cell differentiation in thymus
GO:0034446 substrate adhesion-dependent cell spreading
GO:0035412 regulation of catenin import into nucleus
GO:0042327 positive regulation of phosphorylation
GO:0042666 negative regulation of ectodermal cell fate specification
GO:0045177 apical part of cell
GO:0045941 positive regulation of transcription
GO:0046330 positive regulation of JNK cascade
GO:0048103 somatic stem cell division
GO:0060054 positive regulation of epithelial cell proliferation involved in wound healing
GO:0060070 canonical Wnt receptor signaling pathway
GO:0060231 mesenchymal to epithelial transition
GO:0071300 cellular response to retinoic acid
Regulatory
For research purposes only

Applications of FZD7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 15ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human heart

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000A 50 Tests C P