FYN antibody

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Goat anti FYN

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00019
Datasheet
WB
MSDS
100 µl

Goat anti Human FYN antibody recognizes an epitope within the N-terminal (NT) region of proto-oncogene Tyrosine-protein kinase Fyn encoded by the FYN gene, also known as proto-onocgene Syb or c-Fyn. Human c-Fyn is a 537 amino acid, ~60 kDa, cell membrane non-receptor protein tyrosine kinase and member of the Tyr protein kinase family, SRC subfamily. Fyn contains single protein kinase, SH2 and SH3 domains and shares strong similarities with mouse FYN, v-yes and c-src.

c-Fyn plays roles in many biological processes including cell growth (Yasunaga et al. 1996), adhesion (Chapman et al. 2012), re-modelling (Wang et al. 2012), motility (Yadav et al. 2011) and the immune response (Oykhman et al. 2013).

The immunogen sequence used for the generation of this Goat anti c-Fyn polyclonal antibody is well conserved across a very wide range of mammalian species and the polyclonal anti c-Fyn antibody is expected to have a wide species pattern of reactivity.

Goat anti Human FYN antibody recognizes human FYN as a single band of ~60 kDa in multiple human cell line lysates by western blotting under reducing conditions. It also cross-reacts with rodent cell line lysates tested.

Product Details

Target Species
Human
Western Blotting
Anti FYN antibody detects a band of approximately 60 kDa in HepG2 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-VQCKDKEATKLTE from the N-terminal region of FYN

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P06241
Entrez Gene
FYN
GO Terms
GO:0005886 plasma membrane
GO:0001948 glycoprotein binding
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005768 endosome
GO:0006468 protein phosphorylation
GO:0006816 calcium ion transport
GO:0007243 intracellular protein kinase cascade
GO:0007411 axon guidance
GO:0007612 learning
GO:0007631 feeding behavior
GO:0042802 identical protein binding
GO:0016032 viral reproduction
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0044419 interspecies interaction between organisms
GO:0050690 regulation of defense response to virus by virus
GO:0050852 T cell receptor signaling pathway
GO:0050900 leukocyte migration
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of FYN antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader