FUSE-Binding Protein 1 antibody

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Rabbit anti FUSE-Binding Protein 1

FUSE-binding protein 1 antibody recognizes far upstream element-binding protein 1, also known as FUBP1, DNA helicase V or hDH V. This antibody has been extensively validated for western blotting using whole cell lysates.

FUSE-Binding Protein 1 Antibody with Control Lysate

FUSE-binding protein 1 antibody recognizes far upstream element-binding protein 1, also known as FUBP1, DNA helicase V or hDH V. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00243 WB 100 µl
VPA00243KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human FUSE-binding protein 1 antibody recognizes the far upstream element (FUSE)-binding protein 1, also known as DNA helicase V and hDH V.

Encoded by the FUBP1 gene, FUSE-binding protein 1 is a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase (provided by RefSeq, Jul 2008).

Rabbit anti Human FUSE-binding protein 1 antibody detects a band of 77 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 240-268 amino acids from the central region of human FUSE-binding protein 1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q96AE4 Related reagents
Entrez Gene
FUBP1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003697 single-stranded DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003723 RNA binding
GO:0005634 nucleus
GO:0006366 transcription from RNA polymerase II promoter
GO:0045449 regulation of transcription
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of FUSE-Binding Protein 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti FUSE-binding protein 1 detects a band of approximately 77 kDa in HepG2 cell lysates
Western Blotting
Anti FUSE-binding protein 1 detects a band of approximately 77 kDa in HepG2 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB