Fumarate Hydratase antibody
Goat anti Fumarate Hydratase
Fumarase / fh antibody recognizes fumarate hydratase, also known as Fumarase. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00124 | WB | 100 µl |
Goat anti Human fumarate hydratase antibody recognizes the fumarate hydratase, mitochondrial, also known simply as Fumarase.
Fumarase, encoded by the FH gene, is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in FH can cause fumarase deficiency and lead to progressive encephalopathy (provided by RefSeq, Jul 2008).
Goat anti Human fumarate hydratase antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Fumarase, encoded by the FH gene, is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in FH can cause fumarase deficiency and lead to progressive encephalopathy (provided by RefSeq, Jul 2008).
Goat anti Human fumarate hydratase antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti fumarate hydratase detects a band of approximately 50 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5 % BSA
- Immunogen
- Peptide with the sequence C-HPNDHVNKSQSSND, from the internal region of the protein sequence.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P07954 Related reagents
- Entrez Gene
- FH Related reagents
- GO Terms
- GO:0004333 fumarate hydratase activity
- GO:0005759 mitochondrial matrix
- GO:0006099 tricarboxylic acid cycle
- GO:0006106 fumarate metabolic process
- GO:0030054 cell junction
- GO:0045239 tricarboxylic acid cycle enzyme complex
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Fumarate Hydratase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
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