Frataxin antibody

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Rabbit anti Frataxin

Frataxin antibody recognizes frataxin, also known as Friedreich ataxia protein, FRDA, or FXN.

Frataxin Antibody with Control Lysate

Frataxin antibody recognizes frataxin, also known as Friedreich ataxia protein, FRDA, or FXN.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00877 WB 100 µl
VPA00877KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human frataxin antibody recognizes frataxin, also known as Friedreich ataxia protein, FRDA and FXN.

This nuclear gene encodes a mitochondrial protein thatfunctions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in the autosomal recessive inherited disease Friedreich ataxia. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jun 2009).

Rabbit anti Human frataxin antibody detects a band of 24 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti frataxin antibody recognizes a band of approximately 24 kDa in Raji cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide corresponding to a portion of the C terminal segment of human frataxin
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Raji lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q16595 Related reagents
Entrez Gene
FXN Related reagents
GO Terms
GO:0005515 protein binding
GO:0004322 ferroxidase activity
GO:0005829 cytosol
GO:0005759 mitochondrial matrix
GO:0006783 heme biosynthetic process
GO:0006826 iron ion transport
GO:0006879 cellular iron ion homeostasis
GO:0008198 ferrous iron binding
GO:0008199 ferric iron binding
GO:0008284 positive regulation of cell proliferation
GO:0010039 response to iron ion
GO:0010722 regulation of ferrochelatase activity
GO:0016540 protein autoprocessing
GO:0018283 iron incorporation into metallo-sulfur cluster
GO:0030307 positive regulation of cell growth
GO:0034986 iron chaperone activity
GO:0043066 negative regulation of apoptosis
GO:0048554 positive regulation of metalloenzyme activity
GO:0051347 positive regulation of transferase activity
GO:0051349 positive regulation of lyase activity
GO:0051353 positive regulation of oxidoreductase activity
GO:0051537 2 iron, 2 sulfur cluster binding
GO:0070301 cellular response to hydrogen peroxide
GO:0090201 negative regulation of release of cytochrome c from mitochondria
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Frataxin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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