Frataxin antibody
Rabbit anti Frataxin
Frataxin antibody recognizes frataxin, also known as Friedreich ataxia protein, FRDA, or FXN.
Frataxin Antibody with Control Lysate
Frataxin antibody recognizes frataxin, also known as Friedreich ataxia protein, FRDA, or FXN.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00877 | WB | 100 µl | ||||
| VPA00877KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human frataxin antibody recognizes frataxin, also known as Friedreich ataxia protein, FRDA and FXN.
This nuclear gene encodes a mitochondrial protein thatfunctions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in the autosomal recessive inherited disease Friedreich ataxia. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jun 2009).
Rabbit anti Human frataxin antibody detects a band of 24 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
This nuclear gene encodes a mitochondrial protein thatfunctions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in the autosomal recessive inherited disease Friedreich ataxia. Alternative splicing results in multiple transcript variants (provided by RefSeq, Jun 2009).
Rabbit anti Human frataxin antibody detects a band of 24 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti frataxin antibody recognizes a band of approximately 24 kDa in Raji cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Immunogen
- Synthetic peptide corresponding to a portion of the C terminal segment of human frataxin
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Raji lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q16595 Related reagents
- Entrez Gene
- FXN Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0004322 ferroxidase activity
- GO:0005829 cytosol
- GO:0005759 mitochondrial matrix
- GO:0006783 heme biosynthetic process
- GO:0006826 iron ion transport
- GO:0006879 cellular iron ion homeostasis
- GO:0008198 ferrous iron binding
- GO:0008199 ferric iron binding
- GO:0008284 positive regulation of cell proliferation
- GO:0010039 response to iron ion
- GO:0010722 regulation of ferrochelatase activity
- GO:0016540 protein autoprocessing
- GO:0018283 iron incorporation into metallo-sulfur cluster
- GO:0030307 positive regulation of cell growth
- GO:0034986 iron chaperone activity
- GO:0043066 negative regulation of apoptosis
- GO:0048554 positive regulation of metalloenzyme activity
- GO:0051347 positive regulation of transferase activity
- GO:0051349 positive regulation of lyase activity
- GO:0051353 positive regulation of oxidoreductase activity
- GO:0051537 2 iron, 2 sulfur cluster binding
- GO:0070301 cellular response to hydrogen peroxide
- GO:0090201 negative regulation of release of cytochrome c from mitochondria
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Frataxin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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