Fractalkine antibody

100% Secure

Rabbit anti Human Fractalkine:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP453B E WB 50 µg
Rabbit anti Human Fractalkine antibody recognizes human fractalkine, otherwise known as CX3CL1, a 397 amino acid CX3C chemokine which signals through the CX3CR1 receptor and is expressed by various tissues including the brain, testis, lung, heart, kidney, colon and skeletal muscle.

Adhesion of fractalkine to the CX3CR1 receptor triggers the chemotaxis and adhesion of leucocytes, including T cells, monocytes and NK cells and studies have suggested that fractalkine is involved in the regulation of brain microglia during neuroinflammatory processes (Sunnemark et al. 2005). CX3CL1 may also play a role in dendritic cellmediated host defense against invading pathogens such as Legionella pneumophila (Kikuchi et al. 2005).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Reconstitution
Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin.
Antiserum Preparation
Antisera to human fractalkine were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human fractalkine
Approx. Protein Concentrations
IgG concentration 50ug/ml

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. For long term storage, the addition of 0.09% sodium azide is recommended.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P78423 Related reagents
Entrez Gene
CX3CL1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0006952 defense response
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008009 chemokine activity
GO:0009986 cell surface
GO:0019221 cytokine-mediated signaling pathway
GO:0050729 positive regulation of inflammatory response
GO:0050902 leukocyte adhesive activation
GO:0051041 positive regulation of calcium-independent cell-cell adhesion
Regulatory
For research purposes only

Applications of Fractalkine antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.15 0.30ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

Further Reading

  1. Sunnemark, D. et al. (2005) CX3CL1 (fractalkine) and CX3CR1 expression in myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis: kinetics and cellular origin.
    J Neuroinflammation. 2: 17.
  2. Kikuchi, T. et al. (2005) Involvement of fractalkine/CX3CL1 expression by dendritic cells in the enhancement of host immunity against Legionella pneumophila.
    Infect Immun. 73 (9): 5350-7.