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FOXO4 (pSer197) antibody

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Anti FOXO4 (pSer197) Antibody thumbnail image 1 Anti FOXO4 (pSer197) Antibody thumbnail image 2
Anti FOXO4 (pSer197) Antibody gallery image 1
Western blot analysis of extracts from HEK-293 cells untreated or treated with serum using Rabbit anti FOXO4 (pSer197) antibody. Cells were cultured in serum free media for 16-20 hours, and then cultured in 10% serum culture media for 30 minutes
Anti FOXO4 (pSer197) Antibody gallery image 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti FOXO4 (pSer197) antibody

Rabbit anti FOXO4 (pSer197)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2620
Datasheet
P WB
SDS
50 µl loader

Rabbit anti FOXO4 (pSer197) antibody recognizes FOXO4, when phosphorylated at serine 197. FOXO4 is a member of the forkhead family of transcription factors. Residue 197 of FOXO4 is phosphorylated by a number of kinases including AKT. Phosphorylation of FOXO4 at serine 197 results in decreased transcriptional activity and export of FOXO4 from the nucleus.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to FOXO4 (pSer197) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 197 of human FOXO4

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P98177
Entrez Gene
FOXO4
GO Terms
GO:0000080 G1 phase of mitotic cell cycle
GO:0003690 double-stranded DNA binding
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005829 cytosol
GO:0005667 transcription factor complex
GO:0007050 cell cycle arrest
GO:0007095 mitotic cell cycle G2/M transition DNA damage checkpoint
GO:0009790 embryo development
GO:0007389 pattern specification process
GO:0009888 tissue development
GO:0007517 muscle organ development
GO:0008134 transcription factor binding
GO:0008285 negative regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0008301 DNA bending activity
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016525 negative regulation of angiogenesis
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0019901 protein kinase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030154 cell differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051090 regulation of transcription factor activity
GO:0051151 negative regulation of smooth muscle cell differentiation
Regulatory
For research purposes only

Applications of FOXO4 (pSer197) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

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Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
Request a different product with the FOXO4 (pSer197) specificity