FOXO4 (pSer197) antibody
Rabbit anti FOXO4 (pSer197) antibody recognizes FOXO4, when phosphorylated at serine 197. FOXO4 is a member of the forkhead family of transcription factors. Residue 197 of FOXO4 is phosphorylated by a number of kinases including AKT. Phosphorylation of FOXO4 at serine 197 results in decreased transcriptional activity and export of FOXO4 from the nucleus.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to FOXO4 (pSer197) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 197 of human FOXO4
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P98177 Related reagents
- Entrez Gene
- FOXO4 Related reagents
- GO Terms
- GO:0000080 G1 phase of mitotic cell cycle
- GO:0003690 double-stranded DNA binding
- GO:0003704 specific RNA polymerase II transcription factor activity
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0005829 cytosol
- GO:0005667 transcription factor complex
- GO:0007050 cell cycle arrest
- GO:0007095 mitotic cell cycle G2/M transition DNA damage checkpoint
- GO:0009790 embryo development
- GO:0007389 pattern specification process
- GO:0009888 tissue development
- GO:0007517 muscle organ development
- GO:0008134 transcription factor binding
- GO:0008285 negative regulation of cell proliferation
- GO:0008286 insulin receptor signaling pathway
- GO:0008301 DNA bending activity
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010843 promoter binding
- GO:0016525 negative regulation of angiogenesis
- GO:0016563 transcription activator activity
- GO:0016566 specific transcriptional repressor activity
- GO:0019901 protein kinase binding
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030154 cell differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051090 regulation of transcription factor activity
- GO:0051151 negative regulation of smooth muscle cell differentiation
- Regulatory
- For research purposes only
Applications of FOXO4 (pSer197) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 1/50 | 1/100 | |
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
