Rabbit anti FOXO3A (pSer253) antibody recognizes FOXO3A, when phosphorylated at serine 253. FOXO3A is a member of the forkhead family of transcription factors. Residue 253 of FOXO3A is phosphorylated by a number of kinases including AKT. Phosphorylation of FOXO3A at serine 253 results in decreased transcriptional activity and export of FOXO3A from the nucleus.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to FOXO3A (pSer253) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 253 of human FOXO3A
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
ovulation from ovarian follicle
initiation of primordial ovarian follicle growth
antral ovarian follicle growth
double-stranded DNA binding
specific RNA polymerase II transcription factor activity
sequence-specific enhancer binding RNA polymerase II transcription factor activity
transcription factor complex
induction of apoptosis
pattern specification process
transcription factor binding
insulin receptor signaling pathway
DNA bending activity
negative regulation of gene-specific transcription from RNA polymerase II promoter
transcription activator activity
specific transcriptional repressor activity
protein kinase binding
regulation of cell proliferation
positive regulation of erythrocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
nerve growth factor receptor signaling pathway
regulation of transcription factor activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of FOXO3A (pSer253) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available