FOXO3A antibody

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Rabbit anti FOXO3A

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2245 C E WB 0.1 mg
Rabbit anti FOXO3A antibody recognises human forkhead box, also known as O3A FOXO3A, FKHLR1 and FOXO3, a ~95 kDa member of the forkhead family of transcription factors. FOXO3A is ubiquitously expressed and is an important regulator of apoptosis and the cell cycle.
FOXO3A triggers apoptosis by inducing the expression of genes necessary for cell death.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to Human FOXO3A were raised by repeated immunisation of Rabbits with highly purified antigen Purified IgG prepared from whole serum by affinity chromatography
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to 18 amino acid sequence from near the amino terminus of human FOXO3A.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O43524 Related reagents
Entrez Gene
FOXO3 Related reagents
GO Terms
GO:0001542 ovulation from ovarian follicle
GO:0001544 initiation of primordial ovarian follicle growth
GO:0001547 antral ovarian follicle growth
GO:0001556 oocyte maturation
GO:0003690 double-stranded DNA binding
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005667 transcription factor complex
GO:0006917 induction of apoptosis
GO:0009790 embryo development
GO:0007389 pattern specification process
GO:0009888 tissue development
GO:0008134 transcription factor binding
GO:0008286 insulin receptor signaling pathway
GO:0008301 DNA bending activity
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0019901 protein kinase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0042127 regulation of cell proliferation
GO:0042593 glucose homeostasis
GO:0045648 positive regulation of erythrocyte differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051090 regulation of transcription factor activity
Regulatory
For research purposes only

Applications of FOXO3A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen 4ug/ml
Western Blotting 0.5-1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product is suitable for use in indirect ELISA applications.
Histology Positive Control Tissue
A-20 cells
Western Blotting
AHP2245 detects a band of approximately 96 kDa in A-20 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*