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FOXO3A antibody

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Staining of formalin fixed, paraffin embedded human skin with Rabbit anti Human FOXO3A (AHP2101).

Rabbit anti FOXO3A

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
FOXO3A

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
AHP2101
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Rabbit anti FOXO3A antibody recognises human forkhead box O3A (FOXO3A), also known as FKHLR1 and FOXO3, a ~95 kDa member of the forkhead family of transcription factors.

FOXO3A is ubiquitously expressed and is an important regulator of apoptosis and the cell cycle. FOXO3A triggers apoptosis by inducing the expression of genes necessary for cell death.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Xenopus Expected from Sequence
Rat Expected from Sequence
Monkey Expected from Sequence
Chicken Expected from Sequence
Dog Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to FOXO3A were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide corresponding to a portion of the amino acid sequence between 600-673 of human FOXO3A – KLH conjugated.
Approx. Protein Concentrations
Total protein concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 10ug/ml
Western Blotting 2 4ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Human skin

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10x Antigen Retrieval Buffer, pH8.0 BUF025B P 500 ml
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Description 10x Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
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Description TidyBlot Western Blot Detection Reagent:HRP

Synonyms
Forkhead Box Protein O3
RRID
AB_2263010
UniProt
O43524
Entrez Gene
FOXO3
GO Terms
GO:0001542 ovulation from ovarian follicle
GO:0001544 initiation of primordial ovarian follicle growth
GO:0001547 antral ovarian follicle growth
GO:0001556 oocyte maturation
GO:0003690 double-stranded DNA binding
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005667 transcription factor complex
GO:0006917 induction of apoptosis
GO:0009790 embryo development
GO:0007389 pattern specification process
GO:0009888 tissue development
GO:0008134 transcription factor binding
GO:0008286 insulin receptor signaling pathway
GO:0008301 DNA bending activity
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0019901 protein kinase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0042127 regulation of cell proliferation
GO:0042593 glucose homeostasis
GO:0045648 positive regulation of erythrocyte differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051090 regulation of transcription factor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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