FOXO3A antibody
Rabbit anti FOXO3A antibody recognises human forkhead box O3A (FOXO3A), also known as FKHLR1 and FOXO3, a ~95 kDa member of the forkhead family of transcription factors.
FOXO3A is ubiquitously expressed and is an important regulator of apoptosis and the cell cycle. FOXO3A triggers apoptosis by inducing the expression of genes necessary for cell death.
FOXO3A is ubiquitously expressed and is an important regulator of apoptosis and the cell cycle. FOXO3A triggers apoptosis by inducing the expression of genes necessary for cell death.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Expected from Sequence Xenopus Expected from Sequence Rat Expected from Sequence Monkey Expected from Sequence Chicken Expected from Sequence Dog Expected from Sequence Mouse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to FOXO3A were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide corresponding to a portion of the amino acid sequence between 600-673 of human FOXO3A – KLH conjugated.
- Approx. Protein Concentrations
- Total protein concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O43524
- Entrez Gene
- FOXO3
- GO Terms
- GO:0001542 ovulation from ovarian follicle
- GO:0001544 initiation of primordial ovarian follicle growth
- GO:0001547 antral ovarian follicle growth
- GO:0001556 oocyte maturation
- GO:0003690 double-stranded DNA binding
- GO:0003704 specific RNA polymerase II transcription factor activity
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0005667 transcription factor complex
- GO:0006917 induction of apoptosis
- GO:0009790 embryo development
- GO:0007389 pattern specification process
- GO:0009888 tissue development
- GO:0008134 transcription factor binding
- GO:0008286 insulin receptor signaling pathway
- GO:0008301 DNA bending activity
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0016566 specific transcriptional repressor activity
- GO:0019901 protein kinase binding
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0042127 regulation of cell proliferation
- GO:0042593 glucose homeostasis
- GO:0045648 positive regulation of erythrocyte differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051090 regulation of transcription factor activity
- Regulatory
- For research purposes only
Applications of FOXO3A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 10ug/ml | ||
| Western Blotting | 2 | 4ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
- Human skin
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| 10x Antigen Retrieval Buffer, pH8.0 | BUF025B | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | ||
| Histar Detection System | STAR3000A | C P | 50 Tests |
Fluorescent Spectraviewer
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- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
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- Select combined or multi-laser view to visualize the spectra
