FOXO1A (pSer256) antibody
Rabbit anti FOXO1A (pSer256) antibody recognizes FOXO1A, when phosphorylated at serine 256. FOXO1A is a member of the forkhead family of transcription factors. Residue 256 of FOXO1A is phosphorylated by a number of kinases including AKT. Phosphorylation of FOXO1A at serine 256 results in decreased transcriptional activity and export of FOXO1A from the nucleus (Arden, 2004).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to FOXO1A (pSer256) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 256 of human FOXO1A
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q12778
- Entrez Gene
- FOXO1
- GO Terms
- GO:0001568 blood vessel development
- GO:0003690 double-stranded DNA binding
- GO:0003704 specific RNA polymerase II transcription factor activity
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0005829 cytosol
- GO:0005667 transcription factor complex
- GO:0006916 anti-apoptosis
- GO:0006974 response to DNA damage stimulus
- GO:0009790 embryo development
- GO:0007389 pattern specification process
- GO:0009888 tissue development
- GO:0008134 transcription factor binding
- GO:0008286 insulin receptor signaling pathway
- GO:0008301 DNA bending activity
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0016566 specific transcriptional repressor activity
- GO:0019901 protein kinase binding
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0031018 endocrine pancreas development
- GO:0032873 negative regulation of stress-activated MAPK cascade
- GO:0042127 regulation of cell proliferation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051090 regulation of transcription factor activity
- Regulatory
- For research purposes only
Applications of FOXO1A (pSer256) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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