FOXO1 antibody

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Anti FOXO1 Antibody gallery image 1 — Western blot analysis of FOXO1 was performed by loading 25 ug of HeLa (lane 1), 293T (lane 2) and rat heart lysates (lane 3) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with FOXO1 polyclonal antibody (**AHP2400**) at a dilution of 1/500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Detection was performed using chemiluminescence. Images were taken at an exposure time of 2 min (lanes 1 and 2) and 1 min (lane 3). Results show a band at ~66 kDa.

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Rabbit anti FOXO1

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: FOXO1

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AHP2400 Datasheet Datasheet SDS SDS	WB	50 µg		Log in	Get Quote
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Rabbit anti FOXO1 antibody recognizes Forkhead box protein O1, also known as FOXO1, Forkhead box protein O1A or Forkhead in rhabdomyosarcoma.

FOXO1 like other members of the forkhead protein family acts as a transcription factor by binding to DNA with its forkhead box protein domain. The subcellular activation and thereby the transcriptional activity of FOXO1 is regulated via AKT mediated phosphorylation while its levels are regulated via polyubiquitination.

FOXO1 has been shown to play a role in various signaling pathways ranging from apoptosis, proliferation, oxidative stress, DNA damage to insulin signaling (Huang and Tindall 2007). The protein has also been proposed to act as a tumor suppressor (Fu and Tindall 2008, Xie et al..2012).

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to FOXO1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
1 mg/ml Bovine Serum Albumin
Immunogen: Synthetic peptide corresponding to aa 633-647 of human FOXO1
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Rat

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/100	1/1000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Rabbit anti FOXO1 antibody recognizes a band of approximately 66 kDa in HeLa cell lysate, 293T cell lysate and rat heart tissue lysate

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Synonyms: FKHR; FOXO1A
UniProt: Q12778
Entrez Gene: FOXO1
GO Terms: GO:0001568 blood vessel development; GO:0003690 double-stranded DNA binding; GO:0003704 specific RNA polymerase II transcription factor activity; GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity; GO:0005829 cytosol; GO:0005667 transcription factor complex; GO:0006916 anti-apoptosis; GO:0006974 response to DNA damage stimulus; GO:0009790 embryo development; View More GO Terms; GO:0007389 pattern specification process; GO:0009888 tissue development; GO:0008134 transcription factor binding; GO:0008286 insulin receptor signaling pathway; GO:0008301 DNA bending activity; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010843 promoter binding; GO:0016563 transcription activator activity; GO:0016566 specific transcriptional repressor activity; GO:0019901 protein kinase binding; GO:0048015 phosphatidylinositol-mediated signaling; GO:0031018 endocrine pancreas development; GO:0032873 negative regulation of stress-activated MAPK cascade; GO:0042127 regulation of cell proliferation; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051090 regulation of transcription factor activity