Follistatin antibody

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Rabbit anti Human Follistatin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1280
Datasheet
E WB
MSDS
0.1 mg loader

Rabbit anti Human Follistatin antibody recognizes human follistatin (FST), a single-chain glycosylated secreted protein of gonadal origin, originally identified as an antagonist of activin and suppressor of follicle stimulating hormone (FSH) synthesis/secretion.

As well as activin, FST interacts with and inhibits other members of the transforming growth factor beta (TGF-beta) superfamily, such as bone morphogenetic proteins (BMPs) -2, -4 and -7, myostatin and growth differentiation factor 9 (GDF-9), making FST a key regulator in development and regeneration processes.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to human follistatin were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.
Immunogen
Recombinant human follistatin
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
6 months from date of reconstitution.

More Information

UniProt
P19883 Related reagents
Entrez Gene
FST Related reagents
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005515 protein binding
GO:0002244 hemopoietic progenitor cell differentiation
GO:0005576 extracellular region
GO:0004871 signal transducer activity
GO:0046882 negative regulation of follicle-stimulating hormone secretion
GO:0032926 negative regulation of activin receptor signaling pathway
GO:0048185 activin binding
GO:0051798 positive regulation of hair follicle development
Regulatory
For research purposes only

Applications of Follistatin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Lin, S.Y. et al. (2003) Regulation of ovarian function by the TGF-beta superfamily and follistatin.
    Reproduction. 126 (2): 133-48.