Focal Adhesion Kinase (pTyr861) antibody

100% Secure


Rabbit anti Focal Adhesion Kinase (pTyr861)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2616 WB 50 µl
Rabbit anti focal adhesion kinase (pTyr861) antibody recognizes focal adhesion kinase, also known as FAK, when phosphorylated at tyrosine 861. Focal adhesion kinase is a highly conserved tyrosine kinase with roles in several biological processes including adhesion, migration and cell survival. FAK consists of four domains, an N-terminal FERM domain; a catalytic tyrosine kinase domain; a C-terminal focal-adhesion targeting (FAT) domain and an unstructured proline rich region between the catalytic and FAT domains. Phosphorylation of FAK at tyrosine 861 regulates interactions with the scaffold protein p130cas and contributes to oncogenic transformation of cells in vitro (Lim et al. 2004).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to focal adhesion kinase (pTyr861) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 861 of human focal adhesion kinase

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q05397 Related reagents
Entrez Gene
PTK2 Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0004871 signal transducer activity
GO:0005524 ATP binding
GO:0005856 cytoskeleton
GO:0005925 focal adhesion
GO:0006468 protein phosphorylation
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007172 signal complex assembly
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042169 SH2 domain binding
Regulatory
For research purposes only

Applications of Focal Adhesion Kinase (pTyr861) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Lim, Y. et al. (2004) Phosphorylation of focal adhesion kinase at tyrosine 861 is crucial for Ras transformation of fibroblasts.
    J Biol Chem. 279 (28): 29060-5.