Rabbit anti focal adhesion kinase (pTyr397) antibody
recognizes focal adhesion kinase, also known as FAK, when phosphorylated at tyrosine 397. Focal adhesion kinase is a highly conserved tyrosine kinase with roles in several biological processes including adhesion, migration and cell survival. FAK consists of four domains, an N-terminal FERM domain; a catalytic tyrosine kinase domain; a C-terminal focal-adhesion targeting (FAT) domain and an unstructured proline rich region between the catalytic and FAT domains. Autophosphorylation of FAK at tyrosine 397 occurs in response to integrin activation. Autophosphorylation of FAK at tyrosine 397 causes activation of PLC-gamma1 at sites of focal adhesion (Zhang et al., 1999
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to focal adhesion kinase (pTyr397) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding tyrosine 397 of human focal adhesion kinase
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
non-membrane spanning protein tyrosine kinase activity
signal transducer activity
cellular component disassembly involved in apoptosis
signal complex assembly
integrin-mediated signaling pathway
SH2 domain binding
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Focal Adhesion Kinase (pTyr397) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available