Rabbit anti Human Focal Adhesion Kinase antibody
recognizes human focal adhesion kinase 1, also known as Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71 or Protein-tyrosine kinase 2. Focal adhesion kniase belongs to the Tyr protein kinase family and contains a single FERM
domain and a single protein kinase
domain. Rabbit anti Human Focal Adhesion Kinase antibody detects an epitope within the internal region of Focal adhesion kinase (FAK), a 125 kDa cytoplasmic protein tyrosine kinase that is activated by numerous stimuli and controls cell motility, proliferation and apoptosis. FAK resides at focal adhesions that form between cells growing in the presence of extracellular matrix constituents (Brami-Cherrier et al. 2014
). FAK is a highly conserved molecule amongst vertebrate species and the immunogen sequence domonstrates 100% identity with mouse, rat, many non-human primates, bovine, rabbit and chicken species.
FAK plays a potential role in oncogenic transformation, and its expression is thought to be correlated to tumour invasiveness (Ibbetson et al. 2013
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Focal Adhesion Kinase antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Ductal and lobular epithelium.