Focal Adhesion Kinase antibody

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Rabbit anti Human Focal Adhesion Kinase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1106 P* 50 µg
Rabbit anti Human Focal Adhesion Kinase antibody recognizes human focal adhesion kinase 1, also known as Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71 or Protein-tyrosine kinase 2. Focal adhesion kniase belongs to the Tyr protein kinase family and contains a single FERM domain and a single protein kinase domain. Rabbit anti Human Focal Adhesion Kinase antibody detects an epitope within the internal region of Focal adhesion kinase (FAK), a 125 kDa cytoplasmic protein tyrosine kinase that is activated by numerous stimuli and controls cell motility, proliferation and apoptosis. FAK resides at focal adhesions that form between cells growing in the presence of extracellular matrix constituents (Brami-Cherrier et al. 2014). FAK is a highly conserved molecule amongst vertebrate species and the immunogen sequence domonstrates 100% identity with mouse, rat, many non-human primates, bovine, rabbit and chicken species.

FAK plays a potential role in oncogenic transformation, and its expression is thought to be correlated to tumour invasiveness (Ibbetson et al. 2013).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to focal adhesion kinase were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Synthetic 16 amino acid peptide for the internal domain of human focal adhesion kinase.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q05397 Related reagents
Entrez Gene
PTK2 Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0004871 signal transducer activity
GO:0005524 ATP binding
GO:0005856 cytoskeleton
GO:0005925 focal adhesion
GO:0006468 protein phosphorylation
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007172 signal complex assembly
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042169 SH2 domain binding
Regulatory
For research purposes only

Applications of Focal Adhesion Kinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2 5ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Ductal and lobular epithelium.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for Focal Adhesion Kinase antibody

  1. Mclean, G.W. et al. (2005) The role of focal-adhesion kinase in cancer - a new therapeutic opportunity.
    Nat Rev Cancer. 5 (7): 505-15.
  2. Mitra, S.K. et al. (2005) Focal adhesion kinase: in command and control of cell motility.
    Nat Rev Mol Cell Biol. 6 (1): 56-68.