Flt3 Ligand antibody

Rabbit anti Human Flt3 Ligand

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Flt3 Ligand

Product Code Applications Pack Size List Price Your Price Qty
AHP770
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Human Flt3 Ligand antibody recognizes the ubiquitously expressed human growth factor Flt3-Ligand (Flt3L), a 235aa type I transmembrane glycoprotein which regulates the proliferation of early haematopoietic cells.

Flt3L exists in both a membrane-bound and proteolytically cleaved soluble isoform, both of which are biologically active, and initiates cell proliferation/ differentiation signalling in co-operation with other colony stimulating factors (CSFs) and interleukins, by binding to and activating the tyrosine kinase receptor Flt3 (FLT3R), expressed highly by hematopoietic progenitor cells and stem cells.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
Antiserum Preparation
Antisera to human Flt3-Ligand were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human Flt3-Ligand.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 0.25 0.75ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Graddis, T.J. et al. (1998) Structure-function analysis of FLT3 ligand-FLT3 receptor interactions using a rapid functional screen.
    J Biol Chem. 273 (28): 17626-33.
  2. Wodnar-filipowicz, A. et al. (1996) Flt3 ligand level reflects hematopoietic progenitor cell function in aplastic anemia and chemotherapy-induced bone marrow aplasia.
    Blood. 88 (12): 4493-9.

RRID
AB_2294114
UniProt
P49771
Entrez Gene
FLT3LG
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0007165 signal transduction
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0008284 positive regulation of cell proliferation
View more products with FLT3 LIGAND specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products