FLOTILLIN-2 antibody

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Rabbit anti FLOTILLIN-2

Flotillin-2 antibody recognizes flotillin-2, also known as epidermal surface antigen-flotillin-2 or membrane component chromosome 17 surface marker 1.

FLOTILLIN-2 Antibody with Control Lysate

Flotillin-2 antibody recognizes flotillin-2, also known as epidermal surface antigen-flotillin-2 or membrane component chromosome 17 surface marker 1.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00740 WB 100 µl
VPA00740KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human flotillin-2 antibody recognizes flotillin-2, also known as epidermal surface antigen-flotillin-2 or membrane component chromosome 17 surface marker 1.

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT2 encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling (provided by RefSeq, Jul 2008).

Rabbit anti Human flotillin-2 antibody detects a band of 49 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
1% Bovine Serum Albumin
<20% Glycerol
Preservative Stabilisers
0.01% Thiomersal
1% Bovine Serum Albumin
<20% Glycerol
Immunogen
Synthetic peptide encompassing part of the C terminal region of flotillin-2

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q14254 Related reagents
Entrez Gene
FLOT2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0005624 membrane fraction
GO:0005768 endosome
GO:0005901 caveola
GO:0008544 epidermis development
GO:0009986 cell surface
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of FLOTILLIN-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti flotillin-2 antibody recognizes a band of approximately 49 kDa in HEK293 cell lysates
Western Blotting
Anti flotillin-2 antibody recognizes a band of approximately 49 kDa in HEK293 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB