FLOTILLIN-2 antibody
Rabbit anti FLOTILLIN-2
Flotillin-2 antibody recognizes flotillin-2, also known as epidermal surface antigen-flotillin-2 or membrane component chromosome 17 surface marker 1.
FLOTILLIN-2 Antibody with Control Lysate
Flotillin-2 antibody recognizes flotillin-2, also known as epidermal surface antigen-flotillin-2 or membrane component chromosome 17 surface marker 1.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00740 | WB | 100 µl | ||||
| VPA00740KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human flotillin-2 antibody recognizes flotillin-2, also known as epidermal surface antigen-flotillin-2 or membrane component chromosome 17 surface marker 1.
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT2 encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling (provided by RefSeq, Jul 2008).
Rabbit anti Human flotillin-2 antibody detects a band of 49 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT2 encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling (provided by RefSeq, Jul 2008).
Rabbit anti Human flotillin-2 antibody detects a band of 49 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti flotillin-2 antibody recognizes a band of approximately 49 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
1% Bovine Serum Albumin
<20% Glycerol - Preservative Stabilisers
- 0.01% Thiomersal
1% Bovine Serum Albumin
<20% Glycerol - Immunogen
- Synthetic peptide encompassing part of the C terminal region of flotillin-2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q14254 Related reagents
- Entrez Gene
- FLOT2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0007155 cell adhesion
- GO:0005624 membrane fraction
- GO:0005768 endosome
- GO:0005901 caveola
- GO:0008544 epidermis development
- GO:0009986 cell surface
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of FLOTILLIN-2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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