FLIP Gamma/Delta antibody
Rabbit anit Human FLIP gamma/delta antibody recognizes the apoptosis related protein designated FLIP (FLICE inhibitory protein) or I-FLICE.
FLIP interacts with the adaptor protein FADD, and with caspase-8 (FLICE) and caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1.
FLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs. Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma. This antibody recognizes the human delta and gamma variants but not the alpha or beta variants.
FLIP is widely expressed in human tissues. Rabbit anti Human FLIP gamma/delta antibody detects FLIP as a band of 25 kDa in human Jurkat, Ramos and Raji cell line lysates.
FLIP interacts with the adaptor protein FADD, and with caspase-8 (FLICE) and caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1.
FLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs. Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma. This antibody recognizes the human delta and gamma variants but not the alpha or beta variants.
FLIP is widely expressed in human tissues. Rabbit anti Human FLIP gamma/delta antibody detects FLIP as a band of 25 kDa in human Jurkat, Ramos and Raji cell line lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti FLIP gamma/delta detects a band of approximately 25 kDa in Jurkat cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to aa191-209 at the C-terminus of human FLIP.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O15519
- Entrez Gene
- CFLAR
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of FLIP Gamma/Delta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
Product Specific References
Further Reading
-
Thome, M. et al. (1997) Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors.
Nature. 386 (6624): 517-21. -
Irmler, M. et al. (1997) Inhibition of death receptor signals by cellular FLIP.
Nature. 388 (6638): 190-5. -
Shu, H.B. et al. (1997) Casper is a FADD- and caspase-related inducer of apoptosis.
Immunity. 6 (6): 751-63. -
Hu, S. et al. (1997) I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis.
J Biol Chem. 272 (28): 17255-7.
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