FKBP1A antibody
Rabbit anti FKBP1A
Rabbit anti-human FKBP1A antibody recognizes peptidyl-prolyl cis-trans isomerase FKBP1A, also known as 12 kDa FK506-binding protein, calstabin 1, immunophilin FKBP12, protein kinase C inhibitor 2 or rotamase.
FKBP1A Antibody with Control Lysate
Rabbit anti-human FKBP1A antibody recognizes peptidyl-prolyl cis-trans isomerase FKBP1A, also known as 12 kDa FK506-binding protein, calstabin 1, immunophilin FKBP12, protein kinase C inhibitor 2 or rotamase.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00601 | WB | 100 µl | ||||
| VPA00601KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human FKBP1A antibody recognizes the peptidyl-prolyl cis-trans isomerase FKBP1A, also known as 12 kDa FK506-binding protein, 12 kDa FKBP, FKBP12-Exip3, PPIase FKBP1A, calstabin 1, immunophilin FKBP12, protein kinase C inhibitor 2 or rotamase.
The protein encoded by the FKBP1A gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to FKBP1A, at least one of which is transcribed (provided by RefSeq, Sep 2008).
Rabbit anti Human FKBP1A gene antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by the FKBP1A gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to FKBP1A, at least one of which is transcribed (provided by RefSeq, Sep 2008).
Rabbit anti Human FKBP1A gene antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti FKBP1A detects a band of approximately 16 kDa in Raji cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the middle region of human FKBP1A
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Raji lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P62942 Related reagents
- Entrez Gene
- FKBP1A Related reagents
- GO Terms
- GO:0003755 peptidyl-prolyl cis-trans isomerase activity
- GO:0042026 protein refolding
- GO:0005829 cytosol
- GO:0004871 signal transducer activity
- GO:0005528 FK506 binding
- GO:0006458 'de novo' protein folding
- GO:0007183 SMAD protein complex assembly
- GO:0014802 terminal cisterna
- GO:0022417 protein maturation by protein folding
- GO:0031398 positive regulation of protein ubiquitination
- GO:0032092 positive regulation of protein binding
- GO:0032513 negative regulation of protein phosphatase type 2B activity
- GO:0032925 regulation of activin receptor signaling pathway
- GO:0034205 beta-amyloid formation
- GO:0034713 type I transforming growth factor beta receptor binding
- GO:0042110 T cell activation
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043206 fibril organization
- GO:0046332 SMAD binding
- GO:0048185 activin binding
- GO:0050776 regulation of immune response
- GO:0055010 ventricular cardiac muscle tissue morphogenesis
- GO:0060314 regulation of ryanodine-sensitive calcium-release channel activity
- GO:0060347 heart trabecula formation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of FKBP1A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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