FKBP1A antibody

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Rabbit anti FKBP1A

Rabbit anti-human FKBP1A antibody recognizes peptidyl-prolyl cis-trans isomerase FKBP1A, also known as 12 kDa FK506-binding protein, calstabin 1, immunophilin FKBP12, protein kinase C inhibitor 2 or rotamase.

FKBP1A Antibody with Control Lysate

Rabbit anti-human FKBP1A antibody recognizes peptidyl-prolyl cis-trans isomerase FKBP1A, also known as 12 kDa FK506-binding protein, calstabin 1, immunophilin FKBP12, protein kinase C inhibitor 2 or rotamase.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00601 WB 100 µl
VPA00601KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human FKBP1A antibody recognizes the peptidyl-prolyl cis-trans isomerase FKBP1A, also known as 12 kDa FK506-binding protein, 12 kDa FKBP, FKBP12-Exip3, PPIase FKBP1A, calstabin 1, immunophilin FKBP12, protein kinase C inhibitor 2 or rotamase.

The protein encoded by the FKBP1A gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to FKBP1A, at least one of which is transcribed (provided by RefSeq, Sep 2008).

Rabbit anti Human FKBP1A gene antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti FKBP1A detects a band of approximately 16 kDa in Raji cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the middle region of human FKBP1A
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Raji lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P62942 Related reagents
Entrez Gene
FKBP1A Related reagents
GO Terms
GO:0003755 peptidyl-prolyl cis-trans isomerase activity
GO:0042026 protein refolding
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005528 FK506 binding
GO:0006458 'de novo' protein folding
GO:0007183 SMAD protein complex assembly
GO:0014802 terminal cisterna
GO:0022417 protein maturation by protein folding
GO:0031398 positive regulation of protein ubiquitination
GO:0032092 positive regulation of protein binding
GO:0032513 negative regulation of protein phosphatase type 2B activity
GO:0032925 regulation of activin receptor signaling pathway
GO:0034205 beta-amyloid formation
GO:0034713 type I transforming growth factor beta receptor binding
GO:0042110 T cell activation
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043206 fibril organization
GO:0046332 SMAD binding
GO:0048185 activin binding
GO:0050776 regulation of immune response
GO:0055010 ventricular cardiac muscle tissue morphogenesis
GO:0060314 regulation of ryanodine-sensitive calcium-release channel activity
GO:0060347 heart trabecula formation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of FKBP1A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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