Fibrinogen antibody

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Sheep anti Human Fibrinogen:FITC

Sheep anti Human Fibrinogen

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4440-8004F IF 1 ml
4440-8004 C E IF 1 ml
Sheep anti Human fibrinogen antibody recognizes human fibrinogen, a complex ~340 kDa hetero-hexameric (di-trimeric) glycoprotein consisting of 3 pairs of α, β and γ chains linked by a series of 29 disulphide bonds (Henschen et al. 1983). The six chains are arranged in such a way that all the N-Terminal ends adjoin to form a central E domain with two trimeric coiled coil structures connecting to outer D domains. Fibrinogen plays an important role in the coagulation process with the D and E domains interacting via the C-Terminal ends of the α chains during fibrin clot cross-linking.

Sheep anti human fibrinogen antibody shows minimal cross-reactivity with related serum proteins. Fibrinogen has been identified as a ferritin binding protein in the horse (Orino et al. 1993). Sheep anti human fibrinogen antibody has been successfully as a capture reagent for ferritin - anti ferritin IgG complexes in horse plasma to evaluate the antibody response to ferritin by ELISA (Takahashi et al. 2013).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Human fibrinogen purified from plasma.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02671 Related reagents
P02675 Related reagents
P02679 Related reagents
Entrez Gene
FGA Related reagents
FGB Related reagents
FGG Related reagents
GO Terms
GO:0002576 platelet degranulation
GO:0030674 protein binding, bridging
GO:0005102 receptor binding
GO:0007165 signal transduction
GO:0005577 fibrinogen complex
GO:0009897 external side of plasma membrane
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0043499 eukaryotic cell surface binding
GO:0051258 protein polymerization
GO:0051592 response to calcium ion
GO:0051087 chaperone binding
GO:0005625 soluble fraction
Regulatory
For research purposes only

Applications of Fibrinogen antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/10 1/100
ELISA 1/5000 1/50000
Immunofluorescence 1/200 1/400
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Application Based External Images

Immunofluorescence

Product Specific References

References for Fibrinogen antibody

  1. Brill, A. et al. (2011) von Willebrand factor-mediated platelet adhesion is critical for deep vein thrombosis in mouse models.
    Blood. 117: 1400-7.
  2. Barrera, V. et al. (2011) Host fibrinogen stably bound to hemozoin rapidly activates monocytes via TLR-4 and CD11b/CD18-integrin: a new paradigm of hemozoin action.
    Blood. 117: 5674-82.
  3. Grainger, D.J. et al. (2004) Apolipoprotein E modulates clearance of apoptotic bodies in vitro and in vivo, resulting in a systemic proinflammatory state in apolipoprotein E-deficient mice.
    J Immunol. 173: 6366-75.
  4. Grainger, D.J. et al. (2001) Suppressing Thrombin Generation is Compatible With the Development of Atherosclerosis in Mice
    Thromb Res. 102: 71-80.
  5. Chien, H.W. (2013) Surface conjugation of zwitterionic polymers to inhibit cell adhesion and protein adsorption.
    Colloids Surf B Biointerfaces. 107: 152-9.
  6. Plskova, J. et al. (2004) Quantitative evaluation of the corneal endothelium in the mouse after grafting.
    Br J Ophthalmol. 88: 1209-16.
  7. Takahashi, K. et al. (2013) The presence of heat-labile factors interfering with binding analysis of fibrinogen with ferritin in horse plasma.
    Acta Vet Scand. 55: 70.
  8. Ozaltin, F. et al. (2013) DGKE variants cause a glomerular microangiopathy that mimics membranoproliferative GN.
    J Am Soc Nephrol. 24: 377-84.
  9. Dmitrieva, N.I. and Burg, M.B. (2014) Secretion of von Willebrand factor by endothelial cells links sodium to hypercoagulability and thrombosis.
    Proc Natl Acad Sci U S A. 111: 6485-90.
  10. Johnsen, D. et al. (2016) Disrupting protein tyrosine phosphatase σ does not prevent sympathetic axonal dieback following myocardial infarction.
    Exp Neurol. 276: 1-4.
  11. Terrell SP et al. (2012) Glomerulonephropathy in aged captive Key Largo woodrats (Neotoma floridana smalli).
    Vet Pathol. 49 (4): 710-6.

Further Reading

  1. Kamath, S. & Lip, G.Y. (2003) Fibrinogen: biochemistry, epidemiology and determinants.
    QJM. 96 (10): 711-29.
  2. Mosesson, M.W. (2005) Fibrinogen and fibrin structure and functions.
    J Thromb Haemost. 3 (8): 1894-904.