FGFR2 antibody
Rabbit anti FGFR2
Rabbit anti-human FGFR2 antibody recognizes fibroblast growth factor receptor 2, also known as BEK fibroblast growth factor receptor, keratinocyte growth factor receptor or CD332. This antibody has been extensively validated for western blotting using whole cell lysates.
FGFR2 Antibody with Control Lysate
Rabbit anti-human FGFR2 antibody recognizes fibroblast growth factor receptor 2, also known as BEK fibroblast growth factor receptor, keratinocyte growth factor receptor or CD332. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00476 | WB | 100 µl | ||||
| VPA00476KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human FGFR2 antibody recognizes fibroblast growth factor receptor 2 (FGFR2), alos known as BEK fibroblast growth factor receptor, keratinocyte growth factor receptor or CD332.
The protein encoded by the FGFR2 gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in FGFR2 are associated with many forms of Craniosynostosis, including Crouzon syndrome (CS), Pfeiffer syndrome (PS), Apert syndrome (APRS), Jackson-Weiss syndrome (JWS), Beare-Stevenson cutis gyrata syndrome (BSTVS), Saethre-Chotzen syndrome (SCS), and Familial scaphocephaly syndrome (FSPC). Multiple alternatively spliced transcript variants encoding different isoforms have been noted for FGFR2 (provided by RefSeq, Jan 2009).
Rabbit anti Human FGFR2 antibody detects a band of 92 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by the FGFR2 gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in FGFR2 are associated with many forms of Craniosynostosis, including Crouzon syndrome (CS), Pfeiffer syndrome (PS), Apert syndrome (APRS), Jackson-Weiss syndrome (JWS), Beare-Stevenson cutis gyrata syndrome (BSTVS), Saethre-Chotzen syndrome (SCS), and Familial scaphocephaly syndrome (FSPC). Multiple alternatively spliced transcript variants encoding different isoforms have been noted for FGFR2 (provided by RefSeq, Jan 2009).
Rabbit anti Human FGFR2 antibody detects a band of 92 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti FGFR2 detects a band of approximately 92 kDa in Jurkat cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the C terminal region of human FGFR2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P21802 Related reagents
- Entrez Gene
- FGFR2 Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of FGFR2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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