FGF 10 antibody

100% Secure


Goat anti Human FGF 10

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1745 E P* WB 0.1 mg
Goat anti Human FGF 10 antibody recognizes human FGF 10 (fibroblast growth factor 10), a member of the heparin-binding growth factors family which act as promoters of cellular differentiation and proliferation, and therefore play key roles in embryonic development, morphogenesis, tissue regeneration and tumour growth and invasion.

FGF 10 signals through binding to the FGF Receptor (FGFR) 2b, and has structural and biological activities similar to that of FGF 7. Defects in FGF 10 are responsible for the autosomal dominant disorders known as lacrimo-auriculo-dento-digital syndrome (LADDS), and aplasia of lacrimal and salivary glands (ALSG).

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to human FGF 10 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human FGF 10
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
O15520 Related reagents
Entrez Gene
FGF10 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000187 activation of MAPK activity
GO:0001823 mesonephros development
GO:0005111 type 2 fibroblast growth factor receptor binding
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0008083 growth factor activity
GO:0008201 heparin binding
GO:0008286 insulin receptor signaling pathway
GO:0009986 cell surface
GO:0010634 positive regulation of epithelial cell migration
GO:0010838 positive regulation of keratinocyte proliferation
GO:0016563 transcription activator activity
GO:0031532 actin cytoskeleton reorganization
GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S
GO:0032781 positive regulation of ATPase activity
GO:0032808 lacrimal gland development
GO:0034394 protein localization at cell surface
GO:0042056 chemoattractant activity
GO:0045739 positive regulation of DNA repair
GO:0045740 positive regulation of DNA replication
GO:0046579 positive regulation of Ras protein signal transduction
GO:0046877 regulation of saliva secretion
GO:0048538 thymus development
GO:0050671 positive regulation of lymphocyte proliferation
GO:0050674 urothelial cell proliferation
GO:0050677 positive regulation of urothelial cell proliferation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0051549 positive regulation of keratinocyte migration
GO:0051789 response to protein stimulus
GO:0060019 radial glial cell differentiation
GO:0060054 positive regulation of epithelial cell proliferation involved in wound healing
GO:0060430 lung saccule development
GO:0060447 bud outgrowth involved in lung branching
GO:0060595 fibroblast growth factor receptor signaling pathway involved in mammary gland specification
GO:0061033 secretion by lung epithelial cell involved in lung growth
GO:0070075 tear secretion
GO:0070371 ERK1 and ERK2 cascade
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0071157 negative regulation of cell cycle arrest
GO:0071338 positive regulation of hair follicle cell proliferation
Regulatory
For research purposes only

Applications of FGF 10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P