FEN1 antibody

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Anti FEN1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with FEN1 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to FEN1 (molecular weight 45 kDa)

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Rabbit anti FEN1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: FEN1

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VPA00267 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human FEN1 antibody recognizes FEN1, also known as DNase IV, flap structure-specific endonuclease 1, maturation factor 1, MF1 and RAD2.

The protein encoded by the FEN1 gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by FEN1. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions (provided by RefSeq, Jul 2008).

Rabbit anti Human FEN1 antibody detects a band of 45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti FEN1 detects a band of approximately 45 kDa in HCT-116 cell lysate.
Product Form: Ig fraction - liquid
Preparation: Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH-conjugated synthetic peptide corresponding to aa 243-272 of human FEN1
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20° avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P39748
Entrez Gene: FEN1
GO Terms: GO:0000084 S phase of mitotic cell cycle; GO:0005515 protein binding; GO:0000722 telomere maintenance via recombination; GO:0003684 damaged DNA binding; GO:0003690 double-stranded DNA binding; GO:0005739 mitochondrion; GO:0004523 ribonuclease H activity; GO:0005654 nucleoplasm; GO:0005730 nucleolus; View More GO Terms; GO:0006284 base-excision repair; GO:0006302 double-strand break repair; GO:0008309 double-stranded DNA specific exodeoxyribonuclease activity; GO:0008409 5'-3' exonuclease activity; GO:0009650 UV protection; GO:0017108 5'-flap endonuclease activity; GO:0048015 phosphatidylinositol-mediated signaling; GO:0032201 telomere maintenance via semi-conservative replication; GO:0043137 DNA replication, removal of RNA primer