FEN1 antibody

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Rabbit anti FEN1

Rabbit anti human FEN1 antibody recognizes flap endonuclease 1, also known as DNase IV, FEN-1, flap structure-specific endonuclease 1, maturation factor 1, MF1 or RAD2. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00267 WB 100 µl
Rabbit anti Human FEN1 antibody recognizes FEN1, also known as DNase IV, flap structure-specific endonuclease 1, maturation factor 1, MF1 and RAD2.

The protein encoded by the FEN1 gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by FEN1. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions (provided by RefSeq, Jul 2008).

Rabbit anti Human FEN1 antibody detects a band of 45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti FEN1 detects a band of approximately 45 kDa in HCT-116 cell lysate
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 243-272 of human FEN1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P39748 Related reagents
Entrez Gene
FEN1 Related reagents
GO Terms
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000722 telomere maintenance via recombination
GO:0003684 damaged DNA binding
GO:0003690 double-stranded DNA binding
GO:0005739 mitochondrion
GO:0004523 ribonuclease H activity
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006284 base-excision repair
GO:0006302 double-strand break repair
GO:0008309 double-stranded DNA specific exodeoxyribonuclease activity
GO:0008409 5'-3' exonuclease activity
GO:0009650 UV protection
GO:0017108 5'-flap endonuclease activity
GO:0048015 phosphatidylinositol-mediated signaling
GO:0032201 telomere maintenance via semi-conservative replication
GO:0043137 DNA replication, removal of RNA primer
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of FEN1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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