FEN1 antibody
Rabbit anti FEN1
Rabbit anti human FEN1 antibody recognizes flap endonuclease 1, also known as DNase IV, FEN-1, flap structure-specific endonuclease 1, maturation factor 1, MF1 or RAD2. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00267 | WB | 100 µl |
Rabbit anti Human FEN1 antibody recognizes FEN1, also known as DNase IV, flap structure-specific endonuclease 1, maturation factor 1, MF1 and RAD2.
The protein encoded by the FEN1 gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by FEN1. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions (provided by RefSeq, Jul 2008).
Rabbit anti Human FEN1 antibody detects a band of 45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by the FEN1 gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by FEN1. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions (provided by RefSeq, Jul 2008).
Rabbit anti Human FEN1 antibody detects a band of 45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti FEN1 detects a band of approximately 45 kDa in HCT-116 cell lysate
- Product Form
- Ig fraction - liquid
- Preparation
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 243-272 of human FEN1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P39748 Related reagents
- Entrez Gene
- FEN1 Related reagents
- GO Terms
- GO:0000084 S phase of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000722 telomere maintenance via recombination
- GO:0003684 damaged DNA binding
- GO:0003690 double-stranded DNA binding
- GO:0005739 mitochondrion
- GO:0004523 ribonuclease H activity
- GO:0005654 nucleoplasm
- GO:0005730 nucleolus
- GO:0006284 base-excision repair
- GO:0006302 double-strand break repair
- GO:0008309 double-stranded DNA specific exodeoxyribonuclease activity
- GO:0008409 5'-3' exonuclease activity
- GO:0009650 UV protection
- GO:0017108 5'-flap endonuclease activity
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0032201 telomere maintenance via semi-conservative replication
- GO:0043137 DNA replication, removal of RNA primer
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of FEN1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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