FEN1 antibody

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Anti Human FEN1 Antibody gallery image 1 — Detection of human Fen1 in whole cell lysates of HeLa and 293T cells (5-50 mcg/lane) by Western blotting with Rabbit anti Human Fen1 (**AHP837**). Immunoprecipitation was with **AHP837** (3μg/mg lysate) or another anti Fen1 polyclonal antibody, subsequent detection of immunoprecipitated Fen1 by Western blotting used Rabbit anti Fen-1 antibody (**AHP837**)

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Rabbit anti Human FEN1

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: FEN1

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AHP837 Datasheet Datasheet SDS SDS	IP WB	0.1 mg		Log in	Get Quote
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Rabbit anti Human FEN1 antibody recognizes human Flap endonuclease 1 also known as Flap structure-specific endonuclease, FEN1, Maturation factor 1 or DNase IV. FEN1 is a 380 amino acid ~45 kDa member of the FEN1 sub family of XPG/RAD2 endonucleases which has an important role in lagging strand DNA synthesis and base excision repair (Kim et al. 1998). It is hypothesised that FEN1 may play a role in the inhibltion of triplet repeat expansion (Vallur and Maizels 2010).

Two isoforms of FEN1 are generated by alternative initiation, isoform 1 against which rabbit anti human FEN1 antibody is generated and a truncated isoform FENMIT, lacking the N-terminal region and having a mitochondrial distribution, FENMIT also lacks DNAase activity (Kazak et al. 2013). Initial results suggest that rabbit anti human FEN1 antibody does not recognize the truncated form of human FEN1.

Fen1 is expressed in the nucleus of a wide variety of organ strusctures notably in those of the hematopoietic system including spleen, lymph nodes and spleen. Rabbit anti human FEN1 antibody recognizes a band of ~45 kDa in whole cell lysates of HeLa and 293T by western blotting and has successfully been used for immunoprecipitation of FEN1 from HeLa cell lysates.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antisera to human FEN1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution: TRIS-citrate/Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Synthetic peptide corresponding to a region within the N terminal domain of human FEN1 (locus link ID 2237)
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse

Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation		2ug/mg protein	10ug/mg protein
Western Blotting		1/2000	1/10000

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP837 detects a band of approximately 42kD in HeLa cell lysates.

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RRID: AB_2102797
UniProt: P39748
Entrez Gene: FEN1
GO Terms: GO:0000084 S phase of mitotic cell cycle; GO:0005515 protein binding; GO:0000722 telomere maintenance via recombination; GO:0003684 damaged DNA binding; GO:0003690 double-stranded DNA binding; GO:0005739 mitochondrion; GO:0004523 ribonuclease H activity; GO:0005654 nucleoplasm; GO:0005730 nucleolus; View More GO Terms; GO:0006284 base-excision repair; GO:0006302 double-strand break repair; GO:0008309 double-stranded DNA specific exodeoxyribonuclease activity; GO:0008409 5'-3' exonuclease activity; GO:0009650 UV protection; GO:0017108 5'-flap endonuclease activity; GO:0048015 phosphatidylinositol-mediated signaling; GO:0032201 telomere maintenance via semi-conservative replication; GO:0043137 DNA replication, removal of RNA primer