FEN1 antibody
Rabbit anti Human FEN1 antibody recognizes human Flap endonuclease 1 also known as Flap structure-specific endonuclease, FEN1, Maturation factor 1 or DNase IV. FEN1 is a 380 amino acid ~45 kDa member of the FEN1 sub family of XPG/RAD2 endonucleases which has an important role in lagging strand DNA synthesis and base excision repair (Kim et al. 1998). It is hypothesised that FEN1 may play a role in the inhibltion of triplet repeat expansion (Vallur and Maizels 2010).
Two isoforms of FEN1 are generated by alternative initiation, isoform 1 against which rabbit anti human FEN1 antibody is generated and a truncated isoform FENMIT, lacking the N-terminal region and having a mitochondrial distribution, FENMIT also lacks DNAase activity (Kazak et al. 2013). Initial results suggest that rabbit anti human FEN1 antibody does not recognize the truncated form of human FEN1.
Fen1 is expressed in the nucleus of a wide variety of organ strusctures notably in those of the hematopoietic system including spleen, lymph nodes and spleen. Rabbit anti human FEN1 antibody recognizes a band of ~45 kDa in whole cell lysates of HeLa and 293T by western blotting and has successfully been used for immunoprecipitation of FEN1 from HeLa cell lysates.
Two isoforms of FEN1 are generated by alternative initiation, isoform 1 against which rabbit anti human FEN1 antibody is generated and a truncated isoform FENMIT, lacking the N-terminal region and having a mitochondrial distribution, FENMIT also lacks DNAase activity (Kazak et al. 2013). Initial results suggest that rabbit anti human FEN1 antibody does not recognize the truncated form of human FEN1.
Fen1 is expressed in the nucleus of a wide variety of organ strusctures notably in those of the hematopoietic system including spleen, lymph nodes and spleen. Rabbit anti human FEN1 antibody recognizes a band of ~45 kDa in whole cell lysates of HeLa and 293T by western blotting and has successfully been used for immunoprecipitation of FEN1 from HeLa cell lysates.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human FEN1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- TRIS-citrate/Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to a region within the N terminal domain of human FEN1 (locus link ID 2237)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P39748
- Entrez Gene
- FEN1
- GO Terms
- GO:0000084 S phase of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000722 telomere maintenance via recombination
- GO:0003684 damaged DNA binding
- GO:0003690 double-stranded DNA binding
- GO:0005739 mitochondrion
- GO:0004523 ribonuclease H activity
- GO:0005654 nucleoplasm
- GO:0005730 nucleolus
- GO:0006284 base-excision repair
- GO:0006302 double-strand break repair
- GO:0008309 double-stranded DNA specific exodeoxyribonuclease activity
- GO:0008409 5'-3' exonuclease activity
- GO:0009650 UV protection
- GO:0017108 5'-flap endonuclease activity
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0032201 telomere maintenance via semi-conservative replication
- GO:0043137 DNA replication, removal of RNA primer
- Regulatory
- For research purposes only
Applications of FEN1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | 2ug/mg protein | 10ug/mg protein | |
| Western Blotting | 1/2000 | 1/10000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP837 detects a band of approximately 42kD in HeLa cell lysates.
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Useful Reagents Available
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|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
