FEN1 antibody

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Rabbit anti Human FEN1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP837
Datasheet
IP WB
MSDS
0.1 mg loader

Rabbit anti Human FEN1 antibody recognizes human Flap endonuclease 1 also known as Flap structure-specific endonuclease, FEN1, Maturation factor 1 or DNase IV. FEN1 is a 380 amino acid ~45 kDa member of the FEN1 sub family of XPG/RAD2 endonucleases which has an important role in lagging strand DNA synthesis and base excision repair (Kim et al. 1998). It is hypothesised that FEN1 may play a role in the inhibltion of triplet repeat expansion (Vallur and Maizels 2010).

Two isoforms of FEN1 are generated by alternative initiation, isoform 1 against which rabbit anti human FEN1 antibody is generated and a truncated isoform FENMIT, lacking the N-terminal region and having a mitochondrial distribution, FENMIT also lacks DNAase activity (Kazak et al. 2013). Initial results suggest that rabbit anti human FEN1 antibody does not recognize the truncated form of human FEN1.

Fen1 is expressed in the nucleus of a wide variety of organ strusctures notably in those of the hematopoietic system including spleen, lymph nodes and spleen. Rabbit anti human FEN1 antibody recognizes a band of ~45 kDa in whole cell lysates of HeLa and 293T by western blotting and has successfully been used for immunoprecipitation of FEN1 from HeLa cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human FEN1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
TRIS-citrate/Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to a region within the N terminal domain of human FEN1 (locus link ID 2237)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P39748
Entrez Gene
FEN1
GO Terms
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000722 telomere maintenance via recombination
GO:0003684 damaged DNA binding
GO:0003690 double-stranded DNA binding
GO:0005739 mitochondrion
GO:0004523 ribonuclease H activity
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006284 base-excision repair
GO:0006302 double-strand break repair
GO:0008309 double-stranded DNA specific exodeoxyribonuclease activity
GO:0008409 5'-3' exonuclease activity
GO:0009650 UV protection
GO:0017108 5'-flap endonuclease activity
GO:0048015 phosphatidylinositol-mediated signaling
GO:0032201 telomere maintenance via semi-conservative replication
GO:0043137 DNA replication, removal of RNA primer
Regulatory
For research purposes only

Applications of FEN1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation 2ug/mg protein 10ug/mg protein
Western Blotting 1/2000 1/10000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP837 detects a band of approximately 42kD in HeLa cell lysates.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml