FCP1 antibody
Rabbit anti FCP1
Rabbit anti-human FCP1 antibody recognizes RNA polymerase II subunit A C-terminal domain phosphatase, also known as transcription factor IIF-associating CTD phosphatase 1 or serine phosphatase FCP1a.
FCP1 Antibody with Control Lysate
Rabbit anti-human FCP1 antibody recognizes RNA polymerase II subunit A C-terminal domain phosphatase, also known as transcription factor IIF-associating CTD phosphatase 1 or serine phosphatase FCP1a.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00440 | WB | 100 µl | ||||
| VPA00440KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human FCP1 antibody recognizes FCP1, also known as RNA polymerase II subunit A C-terminal domain phosphatase, serine phosphatase FCP1a or transcription factor IIF-associating CTD phosphatase 1.
The CTDP1 gene encodes a protein which interacts with the carboxy-terminus of the RAP74 subunit of transcription initiation factor TFIIF, and functions as a phosphatase that processively dephosphorylates the C-terminus of POLR2A (a subunit of RNA polymerase II), making it available for initiation of gene expression. Mutations in CTDP1 are associated with "congenital cataracts, facial dysmorphism and neuropathy syndrome" (CCFDN). Alternatively spliced transcript variants encoding different isoforms have been described for CTDP1 (provided by RefSeq, Feb 2011).
Rabbit anti Human FCP1 antibody detects a band of 104 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The CTDP1 gene encodes a protein which interacts with the carboxy-terminus of the RAP74 subunit of transcription initiation factor TFIIF, and functions as a phosphatase that processively dephosphorylates the C-terminus of POLR2A (a subunit of RNA polymerase II), making it available for initiation of gene expression. Mutations in CTDP1 are associated with "congenital cataracts, facial dysmorphism and neuropathy syndrome" (CCFDN). Alternatively spliced transcript variants encoding different isoforms have been described for CTDP1 (provided by RefSeq, Feb 2011).
Rabbit anti Human FCP1 antibody detects a band of 104 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti FCP1 detects a band of approximately 104 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide corresponding to amino acid 247-276 of human FCP1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q9Y5B0 Related reagents
- Entrez Gene
- CTDP1 Related reagents
- GO Terms
- GO:0003899 DNA-directed RNA polymerase activity
- GO:0005654 nucleoplasm
- GO:0006368 transcription elongation from RNA polymerase II promoter
- GO:0006470 protein dephosphorylation
- GO:0008420 CTD phosphatase activity
- GO:0016032 viral reproduction
- GO:0050434 positive regulation of viral transcription
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of FCP1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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