Rabbit anti ezrin (pTyr353) antibody
recognizes ezrin, also known as villin-2, when phosphorylated at tyrosine 353. Ezrin is a member of the Ezrin Radixin Mosin (ERM) family. Ezrin functions as a linker molecule between the plasma membrane and the cytoskeleton. Phosphorylation of ezrin at tyrosine 353 occurs following treatment of cells with epidermal growth factor. Phosphorylation of ezrin at tyrosine 353 is related to signal survival of PI3K/Akt pathway (Gautreau et al. 1999
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ezrin (pTyr353) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding tyrosine 353 of human ezrin
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
actin filament binding
actin filament bundle assembly
cell adhesion molecule binding
cytoskeletal anchoring at plasma membrane
leukocyte cell-cell adhesion
regulation of cell shape
apical plasma membrane
extrinsic to membrane
membrane to membrane docking
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Ezrin (pTyr353) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Gautreau A et al. (1999) Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway.
Proc Natl Acad Sci U S A. 96 (13): 7300-5.
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