Ezrin (pThr567) antibody
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WB ResetRabbit anti Ezrin (pThr567)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Ezrin (pThr567)
Antibody Quality and Performance Guarantee
| Rabbit anti ezrin (pThr567) antibody recognizes ezrin, also known as villin-2, when phosphorylated at threonine 567. Ezrin is a member of the Ezrin Radixin Moesin (ERM) family. Ezrin functions as a linker molecule between the plasma membrane and the cytoskeleton. Phosphorylation of ezrin at threonine 567 regulates binding to F-actin and the cell membrane (Zhu et al. 2007). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ezrin (pThr567) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding threonine 567 of human ezrin
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Further Reading
-
Zhu, L. et al. (2007) High turnover of ezrin T567 phosphorylation: conformation, activity, and cellular function.
Am J Physiol Cell Physiol. 293 (3): C874-84.
- UniProt
- P15311
- Entrez Gene
- EZR
- GO Terms
- GO:0051015 actin filament binding
- GO:0051017 actin filament bundle assembly
- GO:0050839 cell adhesion molecule binding
- GO:0005829 cytosol
- GO:0005730 nucleolus
- GO:0005884 actin filament
- GO:0007016 cytoskeletal anchoring at plasma membrane
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007411 axon guidance
- View More GO Terms
- GO:0008360 regulation of cell shape
- GO:0016324 apical plasma membrane
- GO:0019898 extrinsic to membrane
- GO:0022614 membrane to membrane docking
- GO:0030175 filopodium
- GO:0030863 cortical cytoskeleton
- GO:0031528 microvillus membrane
- GO:0032587 ruffle membrane
AHP2612
141570
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