Ezrin antibody
Goat anti Ezrin
Ezrin antibody recognizes Ezrin , also known as cytovillin, villin-2 or p81. The antibody has been extensively validated for western blotting using whole cell lysates.
Ezrin Antibody with Control Lysate
Ezrin antibody recognizes Ezrin , also known as cytovillin, villin-2 or p81. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00029 | WB | 100 µl | ||||
| VPA00029KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human ezrin antibody recognizes human ezrin, otherwise known as p81 or villin 2. Ezrin is a 586 amino acid, cytoplasmic peripheral membrane protein containing a single FERM domain and member of the ezrin, radixin, moesin (ERM) protein family, which acts as protein-tyrosine kinase substrate.
Ezrin is enriched in microvilli and other cell surface structures and plays a key role in cell surface structure adhesion, membrane trafficking, motility and organization (Ng et al. 2001). Ezrin acts as a linker protein between the plasma membrane and the actin cytoskeleton, and provides anchorage for cell surface molecules such as ICAM and CD44 (Finnerty et al. 2004). Studies have implicated ezrin in the metastatic capacity of various types of human tumors including breast, prostate, bone, endometrial and colon cancers (Ohtani et al. 1999, Elzaqheid et al. 2008).
Goat anti Human ezrin antibody recognizes human ezrin as a single band of ~80 kDa in multiple human cell line lysates by western blotting under reducing conditions.
Ezrin is enriched in microvilli and other cell surface structures and plays a key role in cell surface structure adhesion, membrane trafficking, motility and organization (Ng et al. 2001). Ezrin acts as a linker protein between the plasma membrane and the actin cytoskeleton, and provides anchorage for cell surface molecules such as ICAM and CD44 (Finnerty et al. 2004). Studies have implicated ezrin in the metastatic capacity of various types of human tumors including breast, prostate, bone, endometrial and colon cancers (Ohtani et al. 1999, Elzaqheid et al. 2008).
Goat anti Human ezrin antibody recognizes human ezrin as a single band of ~80 kDa in multiple human cell line lysates by western blotting under reducing conditions.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ezrin antibody detects a band of approximately 80 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence C-QDYEEKTKKAER from an internal region of ezrin
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P15311 Related reagents
- Entrez Gene
- EZR Related reagents
- GO Terms
- GO:0051015 actin filament binding
- GO:0051017 actin filament bundle assembly
- GO:0050839 cell adhesion molecule binding
- GO:0005829 cytosol
- GO:0005730 nucleolus
- GO:0005884 actin filament
- GO:0007016 cytoskeletal anchoring at plasma membrane
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007411 axon guidance
- GO:0008360 regulation of cell shape
- GO:0016324 apical plasma membrane
- GO:0019898 extrinsic to membrane
- GO:0022614 membrane to membrane docking
- GO:0030175 filopodium
- GO:0030863 cortical cytoskeleton
- GO:0031528 microvillus membrane
- GO:0032587 ruffle membrane
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Ezrin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB | ||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB |
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