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Ezrin antibody

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Western blotting analysis of Human Placenta lysate (35μg protein in RIPA buffer) probed using Goat anti Human ezrin antibody (AHP1394) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

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Goat anti Human Ezrin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Ezrin

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
AHP1394
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Goat anti Human Ezrin antibody recognizes human ezrin, otherwise known as p81 or villin 2, Ezrin is a 586 amino acid ~80kDa cytoplasmic peripheral membrane protein containing a single FERM domain and member of the ezrin, radixin, moesin (ERM) protein family, which acts as protein-tyrosine kinase substrate.

Ezrin is enriched in microvilli and other cell surface structures and plays a key role in cell surface structure adhesion, membrane trafficking, motility and organization (Ng et al. 2001). Ezrin acts as a linker protein between the plasma membrane and the actin cytoskeleton, and provides anchorage for cell surface molecules such as ICAM and CD44 (Finnerty et al. 2004). Ezrin is implicated in the metastatic capacity of various types of human tumors including breast, prostate, bone, endometrial and colon cancers (Ohtani et al. 1999, Elzaqheid et al. 2008).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human ezrin was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-QDYEEKTKKAER from the internal region of ezrin (NP_003370.2).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16,000
Western Blotting 1.0 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1394 detects a band of approximately 80kDa in human placenta and duodenum cell lysates.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

Further Reading

  1. Tsukita, S. & Yonemura, S. (1997) ERM (ezrin/radixin/moesin) family: from cytoskeleton to signal transduction.
    Curr Opin Cell Biol. 9 (1): 70-5.

Synonyms
Villin 2
RRID
AB_2293649
UniProt
P15311
Entrez Gene
EZR
GO Terms
GO:0051015 actin filament binding
GO:0051017 actin filament bundle assembly
GO:0050839 cell adhesion molecule binding
GO:0005829 cytosol
GO:0005730 nucleolus
GO:0005884 actin filament
GO:0007016 cytoskeletal anchoring at plasma membrane
GO:0007159 leukocyte cell-cell adhesion
GO:0007411 axon guidance
GO:0008360 regulation of cell shape
GO:0016324 apical plasma membrane
GO:0019898 extrinsic to membrane
GO:0022614 membrane to membrane docking
GO:0030175 filopodium
GO:0030863 cortical cytoskeleton
GO:0031528 microvillus membrane
GO:0032587 ruffle membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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