Ezrin antibody

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Goat anti Human Ezrin

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1394	E WB			0.1 mg

Goat anti Human Ezrin antibody recognizes human ezrin, otherwise known as p81 or villin 2, Ezrin is a 586 amino acid ~80kDa cytoplasmic peripheral membrane protein containing a single FERM domain and member of the ezrin, radixin, moesin (ERM) protein family, which acts as protein-tyrosine kinase substrate.

Ezrin is enriched in microvilli and other cell surface structures and plays a key role in cell surface structure adhesion, membrane trafficking, motility and organization (Ng et al. 2001). Ezrin acts as a linker protein between the plasma membrane and the actin cytoskeleton, and provides anchorage for cell surface molecules such as ICAM and CD44 (Finnerty et al. 2004). Studies have implicated ezrin in the metastatic capacity of various types of human tumours including breast, prostate, bone, endometrial and colon cancers (Ohtani et al. 1999, Elzaqheid et al. 2008).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Dog	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to human ezrin was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin

Immunogen

Peptide sequence C-QDYEEKTKKAER from the internal region of ezrin (NP_003370.2).

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P15311 Related reagents
Entrez Gene: EZR Related reagents
GO Terms: GO:0051015 actin filament binding; GO:0051017 actin filament bundle assembly; GO:0050839 cell adhesion molecule binding; GO:0005829 cytosol; GO:0005730 nucleolus; GO:0005884 actin filament; GO:0007016 cytoskeletal anchoring at plasma membrane; GO:0007159 leukocyte cell-cell adhesion; GO:0007411 axon guidance; GO:0008360 regulation of cell shape; GO:0016324 apical plasma membrane; GO:0019898 extrinsic to membrane; GO:0022614 membrane to membrane docking; GO:0030175 filopodium; GO:0030863 cortical cytoskeleton; GO:0031528 microvillus membrane; GO:0032587 ruffle membrane
Regulatory: For research purposes only

Applications of Ezrin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/16,000
Western Blotting		1.0	3.0ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1394 detects a band of approximately 80kDa in human placenta and duodenum cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	1 mg	F
Rabbit anti Goat IgG (Fc):HRP	STAR122P	1 mg	C E WB