Ezrin antibody

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Anti Human Ezrin Antibody gallery image 1 — Western blotting analysis of Human Placenta lysate (35μg protein in RIPA buffer) probed using Goat anti Human ezrin antibody (**AHP1394**) at 1.0μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

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Goat anti Human Ezrin

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Ezrin

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References (1)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

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AHP1394 Datasheet Datasheet SDS SDS	E WB	0.1 mg		Log in	Get Quote
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Goat anti Human Ezrin antibody recognizes human ezrin, otherwise known as p81 or villin 2, Ezrin is a 586 amino acid ~80kDa cytoplasmic peripheral membrane protein containing a single FERM domain and member of the ezrin, radixin, moesin (ERM) protein family, which acts as protein-tyrosine kinase substrate.

Ezrin is enriched in microvilli and other cell surface structures and plays a key role in cell surface structure adhesion, membrane trafficking, motility and organization (Ng et al. 2001). Ezrin acts as a linker protein between the plasma membrane and the actin cytoskeleton, and provides anchorage for cell surface molecules such as ICAM and CD44 (Finnerty et al. 2004). Ezrin is implicated in the metastatic capacity of various types of human tumors including breast, prostate, bone, endometrial and colon cancers (Ohtani et al. 1999, Elzaqheid et al. 2008).

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Dog Expected from Sequence; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to human ezrin was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: TRIS buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: Peptide sequence C-QDYEEKTKKAER from the internal region of ezrin (NP_003370.2).
Approx. Protein Concentrations: IgG concentration 0.5mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Dog	Expected from Sequence

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/16,000
Western Blotting		1.0	3.0ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1394 detects a band of approximately 80kDa in human placenta and duodenum cell lysates.

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