EWSR1 antibody

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Goat anti EWSR1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00106
Datasheet
WB
MSDS
100 µl

Goat anti Human EWSR1 antibody recognizes the RNA-binding protein EWS, also known as EWS oncogene and Ewing sarcoma breakpoint region 1 protein.

EWSR1 encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between EWSR1 and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in EWSR1, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of EWSR1 results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14 (provided by RefSeq, Jul 2009).

Goat anti Human EWSR1 antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti EWSR1 detects a band of approximately 95 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-TSYDQSSYSQQNTYG, from the internal region of the protein sequence.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q01844
Entrez Gene
EWSR1
GO Terms
GO:0005886 plasma membrane
GO:0000166 nucleotide binding
GO:0003723 RNA binding
GO:0005516 calmodulin binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006350 transcription
GO:0008270 zinc ion binding
GO:0045449 regulation of transcription
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of EWSR1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader