Rabbit anti estrogen receptor alpha (pSer104) antibody
recognizes estrogen receptor alpha, also known as ER-alpha, when phosphorylated at serine 104. Estrogen receptor alpha is a member of the nuclear receptor superfamily and also functions as a ligand-regulated transcription factor. Estrogen receptor alpha consists of several domains including the transcription activation function-1 domain (AF-1), the DNA-binding domain, the hinge domain and the ligand-binding domain (AF-2). Estrogen receptor alpha is phosphorylated at serine 104 by MAPKs, Cdk2 and GSK3 (Thomas et al. 2008
, Rogatsky et al. 1999
, Medunjanin et al. 2005
). Phosphorylation at serine 104 contributes to agonist independent activation of estrogen receptor alpha.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to estrogen receptor alpha (pSer104) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 104 of human estrogen receptor alpha
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
sequence-specific DNA binding transcription factor activity
zinc ion binding
chromatin remodeling complex
nitric-oxide synthase regulator activity
estrogen receptor activity
response to estradiol stimulus
estrogen response element binding
regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
positive regulation of retinoic acid receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Estrogen Receptor Alpha (pSer104) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Thomas, R.S. et al. (2008) Phosphorylation at serines 104 and 106 by Erk1/2 MAPK is important for estrogen receptor-alpha activity.
J Mol Endocrinol. 40 (4): 173-84.
Rogatsky, I. et al. (1999) Potentiation of human estrogen receptor alpha transcriptional activation through phosphorylation of serines 104 and 106 by the cyclin A-CDK2 complex.
J Biol Chem. 274 (32): 22296-302.
Medunjanin, S. et al. (2005) Glycogen synthase kinase-3 interacts with and phosphorylates estrogen receptor alpha and is involved in the regulation of receptor activity.
J Biol Chem. 280 (38): 33006-14.