Erythropoietin Receptor antibody
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Sheep anti Human erythropoietin receptor antibody recognizes the human Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate erythroblast proliferation and differentiation, and maintain progenitor survival.
Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function.
Sheep anti Human erythropoietin receptor antibody (AHP1825) blocks Iodine125 labelled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cell in neutralization studies (Harris et al. 1992).
Sheep anti Human erythropoietin receptor antibody is reported as suitable for use in immunocytochemistry and for use in functional studies. Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose. The recommended concentration for functional assays is 500ug/ml.
Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function.
Sheep anti Human erythropoietin receptor antibody (AHP1825) blocks Iodine125 labelled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cell in neutralization studies (Harris et al. 1992).
Sheep anti Human erythropoietin receptor antibody is reported as suitable for use in immunocytochemistry and for use in functional studies. Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose. The recommended concentration for functional assays is 500ug/ml.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human EPOR was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Partial cDNA of the extracellular domain of human EPOR expressed as a GST fusion protein in E. Coli.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19235
- P14753
- Entrez Gene
- Epor
- EPOR
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0004900 erythropoietin receptor activity
- GO:0009790 embryo development
- GO:0007420 brain development
- GO:0007507 heart development
- GO:0044419 interspecies interaction between organisms
- GO:0046697 decidualization
- GO:0005887 integral to plasma membrane
- Regulatory
- For research purposes only
Applications of Erythropoietin Receptor antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 50ng/ml | ||
| Immunoprecipitation | 10ug/ml | ||
| Western Blotting | 10ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product is suitable for use in indirect ELISA applications.
- Western Blotting
- AHP1825 detects a 49kDa band of EPOR-GST on SDS-PAGE.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:FITC | STAR88F | C | 1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Product Specific References
References for Erythropoietin Receptor antibody
-
Harris, K.W. et al. (1992) Ligand binding properties of the human erythropoietin receptor extracellular domain expressed in Escherichia coli.
J Biol Chem. 267 (21): 15205-9.
