Erythropoietin Receptor antibody

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Sheep anti Human Erythropoietin Receptor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1825
Datasheet
E IP WB
MSDS
0.1 mg loader

Sheep anti Human erythropoietin receptor antibody recognizes the human Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate erythroblast proliferation and differentiation, and maintain progenitor survival.

Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function.

Sheep anti Human erythropoietin receptor antibody (AHP1825) blocks Iodine125 labelled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cell in neutralization studies (Harris et al. 1992).

Sheep anti Human erythropoietin receptor antibody is reported as suitable for use in immunocytochemistry and for use in functional studies. Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose. The recommended concentration for functional assays is 500ug/ml.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human EPOR was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Partial cDNA of the extracellular domain of human EPOR expressed as a GST fusion protein in E. Coli.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P19235 Related reagents
P14753 Related reagents
Entrez Gene
Epor Related reagents
EPOR Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0004900 erythropoietin receptor activity
GO:0009790 embryo development
GO:0007420 brain development
GO:0007507 heart development
GO:0044419 interspecies interaction between organisms
GO:0046697 decidualization
GO:0005887 integral to plasma membrane
Regulatory
For research purposes only

Applications of Erythropoietin Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 50ng/ml
Immunoprecipitation 10ug/ml
Western Blotting 10ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product is suitable for use in indirect ELISA applications.
Western Blotting
AHP1825 detects a 49kDa band of EPOR-GST on SDS-PAGE.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:FITC STAR88F C 1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Product Specific References

References for Erythropoietin Receptor antibody

  1. Harris, K.W. et al. (1992) Ligand binding properties of the human erythropoietin receptor extracellular domain expressed in Escherichia coli.
    J Biol Chem. 267 (21): 15205-9.