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ERK1 (pThr202) antibody

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Anti ERK1 (pThr202) Antibody thumbnail image 1 Anti ERK1 (pThr202) Antibody thumbnail image 2
Anti ERK1 (pThr202) Antibody gallery image 1
Western blot analysis of extracts from HEK-293 cells untreated or treated with PMA using Rabbit anti ERK1 (pThr202) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with PMA (200 nM) for 30 minutes
Anti ERK1 (pThr202) Antibody gallery image 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti ERK1 (pThr202) antibody

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Rabbit anti ERK1 (pThr202)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
ERK1 (pThr202)

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Product Code Applications Pack Size List Price Your Price Qty
AHP2606
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Rabbit anti ERK1 (pThr202) antibody recognizes ERK1, also known as MAPK3, when phosphorylated at threonine 202. ERK1 is a member of the Mitogen Activated Protein Kinase (MAPK) family. MAPKs constitute a diverse set of signaling molecules. In the case of ERK1, a cascade is initiated by binding activation of a membrane bound receptor which causes activation of a small GTPase, RAS, which in turn activates the protein kinase activity of the RAF protein. RAF in turn phosphorylates and activates MEK. MEK induces phosphorylation of ERK1 at residues threonine 202 and tyrosine 204. Phosphorylation of the residues corresponds to activation of ERK1 and mediation of its diverse biological functions including cell growth, differentiation, motility, and adhesion via its effects on transcription, translation, cytoskeletal rearrangement and cross talk with other signaling pathways

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to ERK1 (pThr202) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding threonine 202 of human ERK1
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P27361
Entrez Gene
MAPK3
GO Terms
GO:0000186 activation of MAPKK activity
GO:0000187 activation of MAPK activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004707 MAP kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006361 transcription initiation from RNA polymerase I promoter
GO:0007049 cell cycle
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008063 Toll signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0030168 platelet activation
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051090 regulation of transcription factor activity
GO:0051403 stress-activated MAPK cascade

AHP2606

141564

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