ErbB4 (pTyr1284) antibody
Rabbit anti ErbB4 (pTyr1284) antibody recognizes ErbB4, also known as receptor tyrosine-protein kinase erbB-4 or tyrosine kinase-type cell surface receptor HER4, when phosphorylated at tyrosine 1284.
ErbB4 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases and plays a key role in the regulation of the nervous system, heart and mammary gland. Tyrosine 1284 of ErbB4 has been identified as a phosphorylation site (Kaushansky et al. 2008).
ErbB4 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases and plays a key role in the regulation of the nervous system, heart and mammary gland. Tyrosine 1284 of ErbB4 has been identified as a phosphorylation site (Kaushansky et al. 2008).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ErbB4 (pTyr1284) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding tyrosine 1284 of human ErbB4
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- Q15303 Related reagents
- Entrez Gene
- ERBB4 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004714 transmembrane receptor protein tyrosine kinase activity
- GO:0004716 receptor signaling protein tyrosine kinase activity
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006350 transcription
- GO:0006468 protein phosphorylation
- GO:0008283 cell proliferation
- GO:0016323 basolateral plasma membrane
- GO:0045449 regulation of transcription
- Regulatory
- For research purposes only
Applications of ErbB4 (pTyr1284) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
Further Reading
-
Kaushansky, A. et al. (2008) System-wide investigation of ErbB4 reveals 19 sites of Tyr phosphorylation that are unusually selective in their recruitment properties.
Chem Biol. 15 (8): 808-17.
