ErbB4 (pTyr1284) antibody

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Anti ErbB4 (pTyr1284) Antibody (Polyclonal Antibody Antibody) thumbnail image 2

Anti ErbB4 (pTyr1284) Antibody (Polyclonal Antibody Antibody) gallery image 1 — Western Blot analysis of HepG2 untreated and EGF treated whole cell lysates probed with (A) Mouse anti ErbB4 antibody (VMA00376) or (B) Rabbit anti ErbB4 (pTyr1284) antibody (1/1,1000, **AHP2605**) followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 695 second Clarity Max or a (B) 1 second Clarity Max exposure. ErbB4 (pTyr1284) has a molecular weight of 160 kDa.

Anti ErbB4 (pTyr1284) Antibody (Polyclonal Antibody Antibody) gallery image 2 — Normalized signal intensity of ErbB4 (pTyr1284) from EGF treated and untreated lysates, treated with (+) or without (-) lambda protein phosphatase. Lambda protein phosphatase dephosphorylates serine, threonine and tyrosine residues. Normalized signal intensity was calculated by dividing the TPN-Normalized signal intensity for each phosphoprotein by the TPN-normalized intensity of the corresponding total protein.
Learn more about TPN Total Protein Normalization.

Rabbit anti ErbB4 (pTyr1284)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP2605 Datasheet	WB SDS	50 µl	Log in		Get Quote

Rabbit anti ErbB4 (pTyr1284) antibody recognizes ErbB4, also known as receptor tyrosine-protein kinase erbB-4 or tyrosine kinase-type cell surface receptor HER4, when phosphorylated at tyrosine 1284.

ErbB4 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases and plays a key role in the regulation of the nervous system, heart and mammary gland. Tyrosine 1284 of ErbB4 has been identified as a phosphorylation site (Kaushansky et al. 2008).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse	Expected from Sequence
Rat	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to ErbB4 (pTyr1284) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Sodium Azide
50% Glycerol

Immunogen

Phospho specific-peptide corresponding to residues surrounding tyrosine 1284 of human ErbB4

Storage Information

Storage: Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: Q15303
Entrez Gene: ERBB4
GO Terms: GO:0005515 protein binding; GO:0016021 integral to membrane; GO:0004714 transmembrane receptor protein tyrosine kinase activity; GO:0004716 receptor signaling protein tyrosine kinase activity; GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0006350 transcription; GO:0006468 protein phosphorylation; GO:0008283 cell proliferation; GO:0016323 basolateral plasma membrane; GO:0045449 regulation of transcription
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of ErbB4 (pTyr1284) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	F IF WB	0.1 mg	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in		Get Quote

Product Specific References

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