ErbB3 (pTyr1328) antibody
Learn more about TPN Total Protein Normalization.
Rabbit anti ErbB3 (pTyr1328) antibody recognizes ErbB3, also known as receptor tyrosine-protein kinase erbB-3 or tyrosine kinase-type cell surface receptor HER3, when phosphorylated at tyrosine 1328.
ErbB3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases and plays a key role in cell proliferation and differentiation.
ErbB3 acts as a receptor for neuregulins and heterodimerizes with ErbB2. ErbB3 contains a phosphorylation site at tyrosine 1328 (Tzouros et al. 2013).
ErbB3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases and plays a key role in cell proliferation and differentiation.
ErbB3 acts as a receptor for neuregulins and heterodimerizes with ErbB2. ErbB3 contains a phosphorylation site at tyrosine 1328 (Tzouros et al. 2013).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ErbB3 (pTyr1328) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding tyrosine 1328 of human ErbB3
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P21860
- Entrez Gene
- ERBB3
- GO Terms
- GO:0004714 transmembrane receptor protein tyrosine kinase activity
- GO:0004716 receptor signaling protein tyrosine kinase activity
- GO:0019838 growth factor binding
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005887 integral to plasma membrane
- GO:0005524 ATP binding
- GO:0005615 extracellular space
- GO:0006468 protein phosphorylation
- GO:0007162 negative regulation of cell adhesion
- GO:0007507 heart development
- GO:0009968 negative regulation of signal transduction
- GO:0014037 Schwann cell differentiation
- GO:0014065 phosphatidylinositol 3-kinase cascade
- GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
- GO:0016323 basolateral plasma membrane
- GO:0021545 cranial nerve development
- GO:0030296 protein tyrosine kinase activator activity
- GO:0042060 wound healing
- GO:0042127 regulation of cell proliferation
- GO:0042803 protein homodimerization activity
- GO:0043235 receptor complex
- GO:0043524 negative regulation of neuron apoptosis
- GO:0046982 protein heterodimerization activity
- GO:0051048 negative regulation of secretion
- GO:0051402 neuron apoptosis
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of ErbB3 (pTyr1328) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Tzouros, M. et al. (2013) Development of a 5-plex SILAC method tuned for the quantitation of tyrosine phosphorylation dynamics.
Mol Cell Proteomics. 12 (11): 3339-49.
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