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ErbB3 (pTyr1328) antibody

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Anti ErbB3 (pTyr1328) Antibody (Polyclonal Antibody Antibody) thumbnail image 1 Anti ErbB3 (pTyr1328) Antibody (Polyclonal Antibody Antibody) thumbnail image 2
Anti ErbB3 (pTyr1328) Antibody (Polyclonal Antibody Antibody) gallery image 1
Western Blot analysis of A431 untreated and EGF treated whole cell lysates probed with (A) Rabbit anti ErbB3 antibody (VPA00167) or (B) Rabbit anti ErbB3 (pTyr1328) antibody (1/1,1000, AHP2604) followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 125 second or a (B) 15 second exposure. ErbB3 (pTyr1328) has a molecular weight of 185 kDa.
Anti ErbB3 (pTyr1328) Antibody (Polyclonal Antibody Antibody) gallery image 2
Normalized signal intensity of ErbB3 (pTyr1328) from EGF treated and untreated lysates, treated with (+) or without (-) lambda protein phosphatase. Lambda protein phosphatase dephosphorylates serine, threonine and tyrosine residues. Normalized signal intensity was calculated by dividing the TPN-Normalized signal intensity for each phosphoprotein by the TPN-normalized intensity of the corresponding total protein.
Learn more about TPN Total Protein Normalization.

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WB

Rabbit anti ErbB3 (pTyr1328)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Format
Purified
Specificity
ErbB3 (pTyr1328)

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Product Code Applications Pack Size List Price Your Price Qty
AHP2604
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µl
List Price Your Price

Rabbit anti ErbB3 (pTyr1328) antibody recognizes ErbB3, also known as receptor tyrosine-protein kinase erbB-3 or tyrosine kinase-type cell surface receptor HER3, when phosphorylated at tyrosine 1328.

ErbB3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases and plays a key role in cell proliferation and differentiation.

ErbB3 acts as a receptor for neuregulins and heterodimerizes with ErbB2. ErbB3 contains a phosphorylation site at tyrosine 1328 (Tzouros et al. 2013).		 PrecisionAb Antibodies

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to ErbB3 (pTyr1328) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Proclin™300
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 1328 of human ErbB3
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
ProClin™ is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg
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Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg
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Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg
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Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg
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Further Reading

  1. Tzouros, M. et al. (2013) Development of a 5-plex SILAC method tuned for the quantitation of tyrosine phosphorylation dynamics.
    Mol Cell Proteomics. 12 (11): 3339-49.

UniProt
P21860
Entrez Gene
ERBB3
GO Terms
GO:0004714 transmembrane receptor protein tyrosine kinase activity
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0019838 growth factor binding
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0005615 extracellular space
GO:0006468 protein phosphorylation
GO:0007162 negative regulation of cell adhesion
GO:0007507 heart development
GO:0009968 negative regulation of signal transduction
GO:0014037 Schwann cell differentiation
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0016323 basolateral plasma membrane
GO:0021545 cranial nerve development
GO:0030296 protein tyrosine kinase activator activity
GO:0042060 wound healing
GO:0042127 regulation of cell proliferation
GO:0042803 protein homodimerization activity
GO:0043235 receptor complex
GO:0043524 negative regulation of neuron apoptosis
GO:0046982 protein heterodimerization activity
GO:0051048 negative regulation of secretion
GO:0051402 neuron apoptosis

AHP2604

141562 152098

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