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ErbB3 antibody

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Anti ErbB3 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1
Anti ErbB3 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Rabbit anti ErbB3 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 24 second exposure. Arrow points to ErbB3 (molecular weight 185 kDa)

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WB

Rabbit anti ErbB3

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
ErbB3

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VPA00167
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Rabbit anti Human ErbB3 antibody recognizes ErbB3 also known as receptor tyrosine-protein kinase erbB-3, proto-oncogene-like protein c-ErbB-3 and tyrosine kinase-type cell surface receptor HER3.

ErbB3 encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of ErbB3 and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized (provided by RefSeq, Jul 2008).

Rabbit anti Human ErbB3 antibody detects a band of 185 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti ErbB3 detects a band of approximately 185 kDa in HepG2 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
ErbB3 fusion protein corresponding to amino acids 1283 to 1323 at the carboxyl end of human ErbB3
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P21860
Entrez Gene
ERBB3
GO Terms
GO:0004714 transmembrane receptor protein tyrosine kinase activity
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0019838 growth factor binding
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0005615 extracellular space
GO:0006468 protein phosphorylation
GO:0007162 negative regulation of cell adhesion
GO:0007507 heart development
GO:0009968 negative regulation of signal transduction
GO:0014037 Schwann cell differentiation
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0016323 basolateral plasma membrane
GO:0021545 cranial nerve development
GO:0030296 protein tyrosine kinase activator activity
GO:0042060 wound healing
GO:0042127 regulation of cell proliferation
GO:0042803 protein homodimerization activity
GO:0043235 receptor complex
GO:0043524 negative regulation of neuron apoptosis
GO:0046982 protein heterodimerization activity
GO:0051048 negative regulation of secretion
GO:0051402 neuron apoptosis

VPA00167

120315

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