Goat anti Human Erb3 antibody
recognizes the human Receptor tyrosine-protein kinase erbB-3, also known as ErbB3, Proto-oncogene-like protein c-ErbB-3 or Tyrosine kinase-type cell surface receptor HER3. ErbB3 is a 1342 amino acid single pass type 1 membrane glycoprotein, a tyrosine protein kinase containing a single protein kinase domain, although this domain has been considered inactive (Citri et al. 2003
) however, the kinase domain is competent to bind ATP for catalysis of autophosphorylation (Shi et al. 2010
). Multiple isoforms of ErbB3 generated by alternative splicing have been described (Katoh et al. 1993
). Goat anti Human Erb3 antibody is generated against a peptide located within the C-terminal region and is expected to recognize membrane bound isoforms 1,4 and 5
but not the short isoforms 2 and 3. ErbB3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases, and has a molecular mass of ~150 kDa. ErbB3 acts as a receptor for neuregulin (Zhu et al. 1995
). Heterodimerization of ErbB3 with other EGFR family members leads to cell proliferation and differentiation (Carraway and Cantley 1994
Amplification of this gene and/or over expression of its protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. In breast cancer it has been shown to dimerize with ErbB2 to drive tumour cell proliferation (Alimandi et al. 1995
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ErbB3 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1088 detects a band of approximately 150kDa in Jurkat cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.