ErbB3 antibody

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Anti ErbB3 (C-Terminal) Antibody gallery image 1 — Western blotting analysis of Jurkat lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using Goat anti Human Erbb3 antibody (**AHP1088**) at 0.1μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Goat anti ErbB3 (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1088	E WB			0.1 mg

Goat anti Human Erb3 antibody recognizes the human Receptor tyrosine-protein kinase erbB-3, also known as ErbB3, Proto-oncogene-like protein c-ErbB-3 or Tyrosine kinase-type cell surface receptor HER3. ErbB3 is a 1342 amino acid single pass type 1 membrane glycoprotein, a tyrosine protein kinase containing a single protein kinase domain, although this domain has been considered inactive (Citri et al. 2003) however, the kinase domain is competent to bind ATP for catalysis of autophosphorylation (Shi et al. 2010). Multiple isoforms of ErbB3 generated by alternative splicing have been described (Katoh et al. 1993). Goat anti Human Erb3 antibody is generated against a peptide located within the C-terminal region and is expected to recognize membrane bound isoforms 1,4 and 5 but not the short isoforms 2 and 3. ErbB3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases, and has a molecular mass of ~150 kDa. ErbB3 acts as a receptor for neuregulin (Zhu et al. 1995). Heterodimerization of ErbB3 with other EGFR family members leads to cell proliferation and differentiation (Carraway and Cantley 1994).

Amplification of this gene and/or over expression of its protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. In breast cancer it has been shown to dimerize with ErbB2 to drive tumour cell proliferation (Alimandi et al. 1995).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat	Expected from Sequence
Mouse	Expected from Sequence
Dog	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human ErbB3 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02%	Sodium Azide
0.5%	Bovine Serum Albumin

Immunogen

Peptide sequence C-HSRLFPKANAQRT corresponding to the C-terminal of ErbB3 isoform 1(NP_001973.2).

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P21860 Related reagents
Entrez Gene: ERBB3 Related reagents
GO Terms: GO:0004714 transmembrane receptor protein tyrosine kinase activity; GO:0004716 receptor signaling protein tyrosine kinase activity; GO:0019838 growth factor binding; GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway; GO:0005887 integral to plasma membrane; GO:0005524 ATP binding; GO:0005615 extracellular space; GO:0006468 protein phosphorylation; GO:0007162 negative regulation of cell adhesion; GO:0007507 heart development; GO:0009968 negative regulation of signal transduction; GO:0014037 Schwann cell differentiation; GO:0014065 phosphatidylinositol 3-kinase cascade; GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade; GO:0016323 basolateral plasma membrane; GO:0021545 cranial nerve development; GO:0030296 protein tyrosine kinase activator activity; GO:0042060 wound healing; GO:0042127 regulation of cell proliferation; GO:0042803 protein homodimerization activity; GO:0043235 receptor complex; GO:0043524 negative regulation of neuron apoptosis; GO:0046982 protein heterodimerization activity; GO:0051048 negative regulation of secretion; GO:0051402 neuron apoptosis
Regulatory: For research purposes only

Applications of ErbB3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/32000
Western Blotting		0.1	0.3ug/ml

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1088 detects a band of approximately 150kDa in Jurkat cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	1 mg	F
Rabbit anti Goat IgG (Fc):HRP	STAR122P	1 mg	C E WB

Product Specific References

References for ErbB3 antibody

Holbro, T. et al. (2003) The ErbB2/ErbB3 heterodimer functions as an oncogenic unit: ErbB2 requires ErbB3 to drive breast tumor cell proliferation.
Proc Natl Acad Sci U S A. Jul 2003; 100: 8933.