ErbB2 (pTyr877) antibody
Rabbit anti ErbB2 (pTyr877) antibody recognizes ErbB2, also known as receptor tyrosine-protein kinase erbB-2, proto-oncogene Neu, tyrosine kinase-type cell surface receptor HER2 or CD340, when phosphorylated at tyrosine 877.
ErbB2 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases and plays a key role in cell proliferation and differentiation.
ErbB2 heterodimerizes with other EGFR family members, however no specific ligand for ErbB2 has been identified (Yarden and Sliwkowski 2001). Overexpression of ErbB2 in breast cancer is associated with poor prognosis.
Phosphorylation of ErbB2 at tyrosine 877 is mediated by SRC (Xu et al. 2007).
ErbB2 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases and plays a key role in cell proliferation and differentiation.
ErbB2 heterodimerizes with other EGFR family members, however no specific ligand for ErbB2 has been identified (Yarden and Sliwkowski 2001). Overexpression of ErbB2 in breast cancer is associated with poor prognosis.
Phosphorylation of ErbB2 at tyrosine 877 is mediated by SRC (Xu et al. 2007).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ErbB2 (pTyr877) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding tyrosine 877 of human ErbB2
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P04626
- Entrez Gene
- ERBB2
- GO Terms
- GO:0003677 DNA binding
- GO:0016021 integral to membrane
- GO:0004716 receptor signaling protein tyrosine kinase activity
- GO:0005006 epidermal growth factor receptor activity
- GO:0043125 ErbB-3 class receptor binding
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0006350 transcription
- GO:0007507 heart development
- GO:0008022 protein C-terminus binding
- GO:0008283 cell proliferation
- GO:0042802 identical protein binding
- GO:0014065 phosphatidylinositol 3-kinase cascade
- GO:0019903 protein phosphatase binding
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0042060 wound healing
- GO:0043235 receptor complex
- GO:0043406 positive regulation of MAP kinase activity
- GO:0045449 regulation of transcription
- GO:0045765 regulation of angiogenesis
- GO:0045785 positive regulation of cell adhesion
- GO:0046777 protein autophosphorylation
- GO:0046982 protein heterodimerization activity
- GO:0048471 perinuclear region of cytoplasm
- GO:0050679 positive regulation of epithelial cell proliferation
- Regulatory
- For research purposes only
Applications of ErbB2 (pTyr877) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/200 | 1/500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Yarden, Y. & Sliwkowski, M.X. (2001) Untangling the ErbB signalling network.
Nat Rev Mol Cell Biol. 2 (2): 127-37. -
Xu, W. et al. (2007) Loss of Hsp90 association up-regulates Src-dependent ErbB2 activity.
Mol Cell Biol. 27 (1): 220-8.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
