ErbB2 (pTyr1221/pTyr1222) antibody

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Rabbit anti ErbB2 (pTyr1221/pTyr1222)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2601 WB 50 µl
Rabbit anti ErbB2 (pTyr1221/pTyr1222) antibody recognizes ErbB2, also known as receptor tyrosine-protein kinase erbB-2, proto-oncogene Neu, tyrosine kinase-type cell surface receptor HER2 or CD340, when phosphorylated at tyrosine 1221/tyrosine 1222.

ErbB2 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases and plays a key role in cell proliferation and differentiation.

ErbB2 heterodimerizes with other EGFR family members, however no specific ligand for ErbB2 has been identified (Yarden and Sliwkowski 2001). Overexpression of ErbB2 in breast cancer is associated with poor prognosis.

ErbB2 is autophosphorylated at tyrosine 1221 and tyrosine 1222 (Ren et al. 2002).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to ErbB2 (pTyr1221/pTyr1222) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine1221/tyrosine1222 of human ErbB2

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P04626 Related reagents
Entrez Gene
ERBB2 Related reagents
GO Terms
GO:0003677 DNA binding
GO:0016021 integral to membrane
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005006 epidermal growth factor receptor activity
GO:0043125 ErbB-3 class receptor binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006350 transcription
GO:0007507 heart development
GO:0008022 protein C-terminus binding
GO:0008283 cell proliferation
GO:0042802 identical protein binding
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0019903 protein phosphatase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0042060 wound healing
GO:0043235 receptor complex
GO:0043406 positive regulation of MAP kinase activity
GO:0045449 regulation of transcription
GO:0045765 regulation of angiogenesis
GO:0045785 positive regulation of cell adhesion
GO:0046777 protein autophosphorylation
GO:0046982 protein heterodimerization activity
GO:0048471 perinuclear region of cytoplasm
GO:0050679 positive regulation of epithelial cell proliferation
Regulatory
For research purposes only

Applications of ErbB2 (pTyr1221/pTyr1222) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Yarden, Y. & Sliwkowski, M.X. (2001) Untangling the ErbB signalling network.
    Nat Rev Mol Cell Biol. 2 (2): 127-37.
  2. Ren, Z. & Schaefer, T.S. (2002) ErbB-2 activates Stat3 alpha in a Src- and JAK2-dependent manner.
    J Biol Chem. 277 (41): 38486-93.